Potassium thiocyanate extract vaccine of Pasteurella multocida and its comparison with conventional bacterin vaccine in mice and goats

被引：0

作者：

Sharma, RK ^{[1
]}

Rahman, H ^{[1
]}

机构：

[1] Assam Agr Univ, Dept Microbiol, Coll Vet Sci, Khanapara 781022, Guwahati, India

来源：

INDIAN JOURNAL OF ANIMAL SCIENCES | 2003年 / 73卷 / 06期

关键词：

bacterin; goat; KSCN extract; mice; Pasteurella multocida; vaccine;

D O I：

暂无

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

Crude potassium thiocyanate (KSCN) extract of Pasteurella multocida serotype A was prepared and used as vaccine in goats and mice KSCN extract consisted of 7 detectable proteins with molecular weight (MW) ranging from 29-205 KDa. The vaccine conferred 100% protection in mice against homologous (Pasteurella multocida A) challenge, but it could protect only 66.67% mice against heterologous (Pasteurella haemolytica A2) challenge. The bacterin vaccine could protect 66.67 and 50.00% of mice against homologous and heterologous challenges, respectively. The serum antibody titre expressed in log 2 scale reached the peak all the third week in goats vaccinated with KSCN (7.33) and bacterin preparation (5.16) respectively. Difference in antibody titre developed by the 2 vaccines was statistically significant (P<0.05).

引用

页码：583 / 586

页数：4

共 17 条

[1]

BAXI KK, 1966, THESIS AGRA U AGRA

[2]

BOLLOG DM, 1991, PROTEIN METHODS

[3]

CONFER AW, 1985, AM J VET RES, V46, P342

[4]

DAVIS DH, 1985, PROGR VET MICROBIOLO, V1

[5]

DURHAM JA, 1986, AM J VET RES, V47, P1946

[6] EXPERIMENTAL IMMUNIZATION OF LAMBS AGAINST PNEUMONIC PASTEURELLOSIS [J].

GILMOUR, NJL ;

MARTIN, WB ;

SHARP, JM ;

THOMPSON, DA ;

WELLS, PW ;

DONACHIE, W .

RESEARCH IN VETERINARY SCIENCE, 1983, 35 (01) :80-86

[7]

Kumar A., 1994, Indian Journal of Comparative Microbiology, Immunology and Infectious Diseases, V15, P23

[8]

LOWRY OH, 1951, J BIOL CHEM, V193, P265

[9] PASTEURELLA-HAEMOLYTICA - PURIFICATION OF SALINE-EXTRACTABLE PROTEINS BY ISOELECTROFOCUSING [J].

MCKINNEY, KL ;

CONFER, AW ;

RUMMAGE, JA ;

GENTRY, MJ ;

DURHAM, JA .

VETERINARY MICROBIOLOGY, 1985, 10 (05) :465-480

[10] DEMONSTRATION OF CROSS-PROTECTION BETWEEN PASTEURELLA-MULTOCIDA TYPE-A AND PASTEURELLA-HAEMOLYTICA, SEROTYPE-1 [J].

MUKKUR, TKS .

INFECTION AND IMMUNITY, 1977, 18 (03) :583-585

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