Comparative Protein Expression Profiles Between Eimeria bovis and Eimeria alabamensis Sporozoites Analyzed by Two-Dimensional Gel Electrophoresis

被引：0
作者：
Alcala-Canto, Yazmin [1 ]
Ibarra Velarde, Froylan [1 ]
Rosario-Cruz, Rodrigo [2 ]
Ramos-Martinez, Espiridon [3 ]
机构：
[1] Univ Nacl Autonoma Mexico, Fac Med Vet & Zootecnia, Dept Parasitol, Mexico City 04510, DF, Mexico
[2] Ctr Nacl Invest Disciplinarias Parasitol Vet, Inst Nacl Invest Forestales Agr & Pecuarias, Jiutepec 62550, Morelos, Mexico
[3] Univ Nacl Autonoma Mexico, Fac Med, Dept Patol Expt, Mexico City 04510, DF, Mexico
来源：
COMPARATIVE PARASITOLOGY | 2010年 / 77卷 / 02期
关键词：
Eimeria bovis; Eimeria alabamensis; pathogenicity; reference map; IN-VITRO; CELLS; VIRULENCE; CATTLE;
D O I：
10.1654/4423.1
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
To seek unknown molecules and pathways that might be responsible for the pathogenicity of ruminant coccidiosis, the protein expression profiles of Eimeria bovis and Eimeria alabamensis were compared by 2-dimensional gel electrophoresis. The average gel of E. bovis contained 938 +/- 132 protein spots having an experimental isoelectric point (pI) range of 4.58 to 7.95 and a mass range of 19.1 to 138.2 kDa. A total of 692 +/- 129 protein spots with pis ranging from 4.39 to 7.84 and masses between 17.6 and 127.8 kDa were included in the average gel of E. alabamensis. Our results provide a valuable starting point to detect differences in protein expression distribution between 2 Eimeria species that cause cattle coccidiosis and may also serve as a useful tool for further investigation of the basis accounting for their sharp difference in virulence.
引用
收藏
页码：214 / 217
页数：4
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