Correlation between Serum Levels of Anti-Endothelial Cell Autoantigen and Anti-Dengue Virus Nonstructural Protein 1 Antibodies in Dengue Patients

被引：17

作者：

Cheng, Hsien-Jen ^{[3
]}

Luo, Yueh-Hsia ^{[3
]}

Wan, Shu-Wen ^{[4
]}

Lin, Chiou-Feng ^{[1
,5
]}

Wang, Shan-Tair ^{[6
]}

Nguyen Thanh Hung ^{[2
]}

Liu, Ching-Chuan ^{[7
]}

Ho, Tzong-Shiann ^{[7
]}

Liu, Hsiao-Sheng ^{[4
]}

Yeh, Trai-Ming ^{[8
]}

Lin, Yee-Shin ^{[4
]}

机构：

[1] Taipei Med Univ, Coll Med, Dept Microbiol & Immunol, Taipei, Taiwan

[2] Childrens Hosp 1, Dept Dengue Hemorrhag Fever, Ho Chi Minh City, Vietnam

[3] Natl Cheng Kung Univ, Coll Med, Inst Basic Med Sci, Tainan 70101, Taiwan

[4] Natl Cheng Kung Univ, Coll Med, Dept Microbiol & Immunol, Tainan 70101, Taiwan

[5] Natl Cheng Kung Univ, Coll Med, Inst Clin Med, Tainan 70101, Taiwan

[6] Natl Cheng Kung Univ, Coll Med, Inst Gerontol, Tainan 70101, Taiwan

[7] Natl Cheng Kung Univ, Coll Med, Dept Pediat, Tainan 70101, Taiwan

[8] Natl Cheng Kung Univ, Coll Med, Dept Med Lab Sci & Biotechnol, Tainan 70101, Taiwan

来源：

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE | 2015年 / 92卷 / 05期

关键词：

MOLECULAR MIMICRY; BYSTANDER ACTIVATION; HEMORRHAGIC-FEVER; ENDOTHELIAL-CELLS; CROSS-REACT; DISEASE; VACCINE; HOST; INFECTION; CYTOKINE;

D O I：

10.4269/ajtmh.14-0162

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

We have previously shown that anti dengue virus nonstructural protein 1 (anti-DENV NS1) antibodies cross-react with endothelial cells, and several autoantigens have been identified. This study shows that the antibody levels against these self-proteins are higher in sera from patients with dengue hemorrhagic fever (DHF) than those in control sera. Anti protein disulfide isomerase (PDI) and anti heat shock protein 60 (anti-HSP60) IgM levels correlated with both anti endothelial cells and anti-DENV NS1 IgM titers. A cross-reactive epitope on the NS1 amino acid residues 311-330 (P311-330) had been predicted. We further found that there were higher IgM and IgG levels against P311-330 in DHF patients' sera than those in the control sera. In addition, correlations were observed between anti-PDI with anti-P31 P311-330 IgM and IgG levels, respectively. Therefore, our results indicate that DENV NS1 P311-330 is a major epitope for cross-reactive antibodies to PDI on the endothelial cell surface, which may play an important role in DENV infection induced autoimmunity.

引用

页码：989 / 995

页数：7

共 70 条

[1] HMEC-1 - ESTABLISHMENT OF AN IMMORTALIZED HUMAN MICROVASCULAR ENDOTHELIAL-CELL LINE [J].

ADES, EW ;

CANDAL, FJ ;

SWERLICK, RA ;

GEORGE, VG ;

SUMMERS, S ;

BOSSE, DC ;

LAWLEY, TJ .

JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1992, 99 (06) :683-690

[2]

[Anonymous], 1997, Dengue hemorrhagic fever: Diagnosis, treatment, prevention and control, V2nd

[3] Lethal Antibody Enhancement of Dengue Disease in Mice Is Prevented by Fc Modification [J].

Balsitis, Scott J. ;

Williams, Katherine L. ;

Lachica, Ruben ;

Flores, Diana ;

Kyle, Jennifer L. ;

Mehlhop, Erin ;

Johnson, Syd ;

Diamond, Michael S. ;

Beatty, P. Robert ;

Harris, Eva .

PLOS PATHOGENS, 2010, 6 (02)

[4] Vascular endothelium: the battlefield of dengue viruses [J].

Basu, Atanu ;

Chaturvedi, Umesh C. .

FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY, 2008, 53 (03) :287-299

[5] The global distribution and burden of dengue [J].

Bhatt, Samir ;

Gething, Peter W. ;

Brady, Oliver J. ;

Messina, Jane P. ;

Farlow, Andrew W. ;

Moyes, Catherine L. ;

Drake, John M. ;

Brownstein, John S. ;

Hoen, Anne G. ;

Sankoh, Osman ;

Myers, Monica F. ;

George, Dylan B. ;

Jaenisch, Thomas ;

Wint, G. R. William ;

Simmons, Cameron P. ;

Scott, Thomas W. ;

Farrar, Jeremy J. ;

Hay, Simon I. .

NATURE, 2013, 496 (7446) :504-507

[6] Hepatitis C-Associated Rheumatic Disorders [J].

Buskila, Dan .

RHEUMATIC DISEASE CLINICS OF NORTH AMERICA, 2009, 35 (01) :111-+

[7] Deletion of the C-Terminal Region of Dengue Virus Nonstructural Protein 1 (NS1) Abolishes Anti-NS1-Mediated Platelet Dysfunction and Bleeding Tendency [J].

Chen, Mei-Chun ;

Lin, Chiou-Feng ;

Lei, Huan-Yao ;

Lin, Shih-Chao ;

Liu, Hsiao-Sheng ;

Yeh, Trai-Ming ;

Anderson, Robert ;

Lin, Yee-Shin .

JOURNAL OF IMMUNOLOGY, 2009, 183 (03) :1797-1803

[8] Anti-dengue virus nonstructural protein 1 antibodies recognize protein disulfide isomerase on platelets and inhibit platelet aggregation [J].

Cheng, Hsien-Jen ;

Lei, Huan-Yao ;

Lin, Chiou-Feng ;

Luo, Yueh-Hsia ;

Wan, Shu-Wen ;

Liu, Hsiao-Sheng ;

Yeh, Trai-Ming ;

Lin, Yee-Shin .

MOLECULAR IMMUNOLOGY, 2009, 47 (2-3) :398-406

[9] Proteomic Analysis of Endothelial Cell Autoantigens Recognized by Anti-Dengue Virus Nonstructural Protein 1 Antibodies [J].

Cheng, Hsien-Jen ;

Lin, Chiou-Feng ;

Lei, Huan-Yao ;

Liu, Hsiao-Sheng ;

Yeh, Trai-Ming ;

Luo, Yueh-Hsia ;

Lin, Yee-Shin .

EXPERIMENTAL BIOLOGY AND MEDICINE, 2009, 234 (01) :63-73

[10] Recent advances in deciphering viral and host determinants of dengue virus replication and pathogenesis [J].

Clyde, Karen ;

Kyle, Jennifer L. ;

Harris, Eva .

JOURNAL OF VIROLOGY, 2006, 80 (23) :11418-11431

← 1 2 3 4 5 6 7 →