Reproducibility of the high-performance liquid chromatographic fingerprints obtained from two soybean cultivars and a selected progeny

被引：15
作者：
Faghihi, J
Jiang, XF
Vierling, R
Goldman, S
Sharfstein, S
Sarver, J
Erhardt, P
机构：
[1] Univ Toledo, Ctr Drug Design & Dev, Toledo, OH 43606 USA
[2] Purdue Univ, Dept Entomol, W Lafayette, IN 47907 USA
[3] Purdue Univ, Dept Agron, W Lafayette, IN 47907 USA
来源：
JOURNAL OF CHROMATOGRAPHY A | 2001年 / 915卷 / 1-2期
关键词：
soybean; roots; phytoalexins;
D O I：
10.1016/S0021-9673(01)00648-3
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Roots from soybean cultivars Williams 82 and Hartwig along with one of their progeny 14a, were extracted with non-polar, moderately polar, and highly polar solvent systems. Extracts were compared by thin-layer chromatography and by HPLC. Methanol extractions conducted at ambient temperature coupled with analysis by reversed-phase HPLC using UV detection provided the most representative sets of reproducible fingerprints. Further optimization of the overall protocol should allow for the profiling of different soybean cultivars when their roots are exposed to various environments and insults during early growth. (C) 2001 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：61 / 74
页数：14
相关论文
共 22 条
[1]   RESISTANCE, SUSCEPTIBILITY AND ACCUMULATION OF GLYCEOLLINS-I-III IN SOYBEAN ORGANS INOCULATED WITH PHYTOPHTHORA-MEGASPERMA F SP-GLYCINEA [J].
BHATTACHARYYA, MK ;
WARD, EWB .
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY, 1986, 29 (02) :227-237
[2]   THIN-LAYER CHROMATOGRAPHY OF APOMORPHINE AND ITS ANALOGS [J].
ERHARDT, PW ;
SMITH, RV ;
SAYTHER, TT ;
KEISER, JE .
JOURNAL OF CHROMATOGRAPHY, 1976, 116 (01) :218-224
[3]   Comprehensive analysis of organic ligands in whole root exudates using nuclear magnetic resonance and gas chromatography mass spectrometry [J].
Fan, TWM ;
Lane, AN ;
Pedler, J ;
Crowley, D ;
Higashi, RM .
ANALYTICAL BIOCHEMISTRY, 1997, 251 (01) :57-68
[4]   GLYCEOLLIN-I IN SOYBEAN-CYST NEMATODE INTERACTIONS - SPATIAL AND TEMPORAL DISTRIBUTION IN ROOTS OF RESISTANT AND SUSCEPTIBLE SOYBEANS [J].
HUANG, JS ;
BARKER, KR .
PLANT PHYSIOLOGY, 1991, 96 (04) :1302-1307
[5]   INDUCIBLY-FORMED ISOFLAVONOIDS FROM LEAVES OF SOYBEAN [J].
INGHAM, JL ;
KEEN, NT ;
MULHEIRN, LJ ;
LYNE, RL .
PHYTOCHEMISTRY, 1981, 20 (04) :795-798
[6]  
Kaufman P B, 1997, J Altern Complement Med, V3, P7, DOI 10.1089/acm.1997.3.7
[7]   SEPARATION AND DETERMINATION OF MUGINEIC ACID AND ITS ANALOGS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].
KAWAI, S ;
SATO, Y ;
TAKAGI, SI ;
NOMOTO, K .
JOURNAL OF CHROMATOGRAPHY, 1987, 391 (01) :325-327
[8]   CHANGES IN MESSENGER-RNA SPECIES DURING DROUGHT STRESS IN WINTER-WHEAT [J].
KING, SW ;
VIERLING, RA ;
NGUYEN, HT .
CROP SCIENCE, 1992, 32 (03) :822-825
[9]   SEPARATION OF GLYCEOLLIN ISOMER-I ISOMER-III BY THIN-LAYER CHROMATOGRAPHY [J].
KOMIVES, T .
JOURNAL OF CHROMATOGRAPHY, 1983, 261 (03) :423-424
[10]   Partial resistance to root-borne infection by Phytophthora sojae in three allelic necrotic root mutants in soybean [J].
Kosslak, RM ;
Dieter, JR ;
Ruff, RL ;
Chamberlin, MA ;
Bowen, BA ;
Palmer, RG .
JOURNAL OF HEREDITY, 1996, 87 (06) :415-422
123