In Situ Formation of Covalent Second Network in a DNA Supramolecular Hydrogel and Its Application for 3D Cell Imaging

被引：56

作者：

Cao, Tianyang ^{[2
]}

Jia, Haoyang ^{[2
]}

Dong, Yuanchen ^{[3
]}

Gui, Songbai ^{[1
]}

Liu, Dongsheng ^{[2
]}

机构：

[1] Capital Med Univ, Beijing Tiantan Hosp, Dept Neurosurg, Beijing 100071, Peoples R China

[2] Tsinghua Univ, Dept Chem, Beijing 100084, Peoples R China

[3] Chinese Acad Sci, Inst Chem, CAS Key Lab Colloid Interface & Chem Thermodynam, 2 Zhongguancun Beiyijie, Beijing 100190, Peoples R China

来源：

ACS APPLIED MATERIALS & INTERFACES | 2020年 / 12卷 / 04期

基金：

中国国家自然科学基金;

关键词：

DNA hydrogels; double-network; mechanical properties; in situ polymerization; 3D imaging; EXTRACELLULAR-MATRIX; PERSISTENCE LENGTHS; GELATION;

D O I：

10.1021/acsami.9b11534

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

DNA hydrogels have been demonstrated with important applications in three-dimensional cell culture in vitro due to their good biocompatibility, biodegradability, and permeability. In these applications, to observe the cell morphology and functions in situ, immobilization, labeling, and imaging processes are involved, which requires good stability of the hydrogels during washing and immersion. To improve the stability of the hydrogels for better imaging, here we built a covalent second network in a DNA supramolecular hydrogel by in situ polymerization and successfully constructed a stable three-dimensional transparent system for cell culture and observation. This strategy has been proved to be efficient in enhancing the mechanical properties and immobilizing the cells inside the hydrogel, which can be applied for immunostaining and cell imaging.

引用

页码：4185 / 4192

页数：8

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