NEK7 is a centrosomal kinase critical for microtubule nucleation

被引：65

作者：

Kim, Sunghwan

Lee, Kwanwoo

Rhee, Kunsoo ^{[1
]}

机构：

[1] Seoul Natl Univ, Dept Biol Sci, Seoul 151742, South Korea

[2] Seoul Natl Univ, Res Ctr Funct Cellulom, Seoul 151742, South Korea

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2007年 / 360卷 / 01期

关键词：

NEK7; mitotic kinases; centrosome; spindle assembly; microtubule nucleation; cytokinesis;

D O I：

10.1016/j.bbrc.2007.05.206

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

NIMA in Aspergillus nidulans is a mitotic kinase for chromosome condensation and segregation. We characterized NEK7, a human homologue of Aspergillus NIMA. NEK7 was located at the centrosome throughout the cell cycle. Temporal localization of NEK7 at midbody of the cytokinetic cell was also observed. NEK7 knockdown by RNAi caused a prometaphase arrest of the cell cycle with monopolar or disorganized spindle. We propose that such defects in spindle assembly are resulted from reduction in microtubule nucleation activity at the centrosome. In consistent to the proposal, we observed a decrease in the centrosomal gamma-tubulin levels and reduction of the microtubule re-growth activity in the NEK7-suppressed cells. In addition, it was evident that NEK7 was directly involved in cytokinesis. (c) 2007 Elsevier Inc. All rights reserved.

引用

页码：56 / 62

页数：7

共 36 条

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