Evidence for contribution of neutral trehalase in barotolerance of Saccharomyces cerevisiae

被引:27
作者
Iwahashi, H [1 ]
Nwaka, S [1 ]
Obuchi, K [1 ]
机构
[1] Natl Inst Biosci & Human Technol, Tsukuba, Ibaraki 3058566, Japan
关键词
D O I
10.1128/AEM.66.12.5182-5185.2000
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
in yeast, trehalose accumulation and its hydrolysis, which is catalyzed by neutral trehalase, are believed to be important for thermotolerance, We have shown that trehalose is one of the important factors for barotolerance (resistance to hydrostatic pressure); however, nothing is known about the role of neutral trehalase in barotolerance. To estimate the contribution of neutral trehalase in resisting high hydrostatic pressure, we measured the barotolerance of neutral trehalase I and/or neutral trehalase II deletion strains. Under 180 MPa of pressure for 2 h, the neutral trehalase I deletion strain showed higher barotolerance in logarithmic-phase cells and lower barotolerance in stationary-phase cells than the wild-type strain, Introduction of the neutral trehalase I gene (NTH1) into the deletion mutant restored barotolerance defects in stationary-phase cells, Furthermore, we assessed the contribution of neutral trehalase during pressure and recovery conditions by varying the expression of NTN1 or neutral trehalase activity with a galactose-inducible GAL1 promoter with either glucose or galactose, The low barotolerance observed with glucose repression of neutral trehalase from the GAL1 promoter was restored during recovery with galactose induction. Our results suggest that neutral trehalase contributes to barotolerance, especially during recovery.
引用
收藏
页码:5182 / 5185
页数:4
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