Urinary F2-isoprostanes in young healthy children at risk for type 1 diabetes mellitus

被引:23
作者
Kauffman, LD
Sokol, RJ
Jones, RH
Awad, JA
Rewers, MJ
Norris, JM
机构
[1] Univ Colorado, Hlth Sci Ctr, Dept Prevent Med & Biometr, Denver, CO 80262 USA
[2] Univ Colorado, Hlth Sci Ctr, Dept Pediat,Pediat Liver Ctr, Sect Pediat Gastroenterol Hepatol & Nutr, Denver, CO 80262 USA
[3] Univ Colorado, Hlth Sci Ctr, Dept Pediat, Liver Transplantat Program, Denver, CO 80262 USA
[4] Childrens Hosp, Denver, CO USA
[5] Vanderbilt Univ, Sch Med, Dept Med, Div Gastroenterol, Nashville, TN 37212 USA
[6] Vanderbilt Univ, Sch Med, Dept Pharmacol, Div Gastroenterol, Nashville, TN 37212 USA
[7] Univ Colorado, Hlth Sci Ctr, Dept Pediat, Denver, CO 80262 USA
[8] Univ Colorado, Hlth Sci Ctr, Barbara Davis Ctr Childhood Diabet, Denver, CO 80262 USA
关键词
MeSH; children; F-2-isoprostanes; oxidative stress; antioxidants; vitamin E; carotenoids; free radicals;
D O I
10.1016/S0891-5849(03)00333-2
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Oxidative stress has been linked to many diseases, but little information exists on biomarkers of oxidative stress in healthy children. The purpose of this study was to describe factors that correlate with urinary F-2-isoprostanes, an indicator of oxidative stress, and to establish normal concentrations of F-2-isoprostanes in children at risk to develop type 1 diabetes mellitus. Creatinine-adjusted urinary F-2-isoprostanes were assessed in 342 Denver children under the age of 7 years, from whom we had collected data during 769 clinic visits from August 1997 through January 2001 (mean 2.3 visits per child). Children were identified by newborn screening for HLA-markers, of varying degrees of prediction, for the development of type 1 diabetes. Plasma antioxidants and carotenoids, age at clinic visit, vitamin supplement use, exposure to environmental tobacco smoke, gender, and race were evaluated as correlates to the degree of oxidative stress, using mixed models for longitudinal data. F-2-isoprostane levels were highest in infancy and decreased nonlinearly until 7 years. Female gender, HLA-DR3/4 genotype, higher plasma gamma-tocopherol: total lipids ratio, and lower alpha-carotene: total lipids ratio correlated with higher F-2-isoprostane levels. Normal values in this healthy population can be used as the basis for future studies of disease mechanisms involving oxidative stress. (C) 2003 Elsevier.
引用
收藏
页码:551 / 557
页数:7
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