Development of Electrochemical Sensor for Homocysteine Determination as a Cerebrovascular Disease Biomarker

This work proposed the glassy carbon electrodes (GCE) based on reduced graphene oxide-TiO2 (RGO-TiO2) nanocomposite to realize sensitive homocysteine (Hcy) detection. The RGO-TiO2 exhibits a remarkable electrocatalytic activity to Hcy oxidation at a low potential, as indicated in cyclic voltammetric (CV) results. The RGO-TiO2-based GCE has also been found highly stable and reproducible. Furthermore, it is strongly resistant to electrode fouling. In addition to these analytical features, it requires low operation potential. Therefore the proposed RGO-TiO2-based GCE could reliably and sensitively detect Hcy, with a limit of detection (LOD) of 24 nM (S/N=3) and a desirable dynamic linearity (80 mu M).