Drosophila FACT contributes to Hox gene expression through physical and functional interactions with GAGA factor

被引：94

作者：

Shimojima, T

Okada, M

Nakayama, T

Ueda, H

Okawa, K

Iwamatsu, A

Handa, H

Hirose, S ^{[1
]}

机构：

[1] Grad Univ Adv Studies, Dept Dev Genet, Natl Inst Genet, Mishima, Shizuoka 4118540, Japan

[2] Grad Univ Adv Studies, Dept Dev Genet, Mishima, Shizuoka 4118540, Japan

[3] Tokyo Inst Technol, Fac Biosci & Biotechnol, Midori Ku, Yokohama, Kanagawa 2268501, Japan

[4] Tokyo Inst Technol, Frontier Collaborat Res Ctr, Midori Ku, Yokohama, Kanagawa 2268501, Japan

[5] Kirin Brewery Co Ltd, Cent Labs Key Technol, Kanazawa Ku, Yokohama, Kanagawa 2360004, Japan

来源：

GENES & DEVELOPMENT | 2003年 / 17卷 / 13期

关键词：

FACT; GAGA factor; Hox gene; chromatin; Drosophila;

D O I：

10.1101/gad.1086803

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Chromatin structure plays a critical role in the regulation of transcription. Drosophila GAGA factor directs chromatin remodeling to its binding sites. We show here that Drosophila FACT (facilitates chromatin transcription), a heterodimer of dSPT16 and dSSRP1, is associated with GAGA factor through its dSSRP1 subunit, binds to a nucleosome, and facilitates GAGA factor-directed chromatin remodeling. Moreover, genetic interactions between Trithorax-like encoding GAGA factor and spt16 implicate the GAGA factor-FACT complex in expression of Hox genes Ultrabithorax, Sex combs reduced, and Abdominal-B. Chromatin immunoprecipitation experiments indicated the presence of the GAGA factor-FACT complex in the regulatory regions of Ultrabithorax and Abdominal-B. These data illustrate a crucial role of FACT in the modulation of chromatin structure for the regulation of gene expression.

引用

页码：1605 / 1616

页数：12

共 54 条

[1] The glutamine-rich domain of the Drosophila GAGA factor is necessary for amyloid fibre formation in vitro, but not for chromatin remodelling [J].