Loss of PRC1 induces higher-order opening of Hox loci independently of transcription during Drosophila embryogenesis

被引:41
作者
Cheutin, Thierry [1 ,2 ]
Cavalli, Giacomo [1 ,2 ]
机构
[1] CNRS, Inst Human Genet, Montpellier, France
[2] Univ Montpellier, Montpellier, France
来源
NATURE COMMUNICATIONS | 2018年 / 9卷
基金
欧洲研究理事会; 欧盟地平线“2020”;
关键词
POLYCOMB RESPONSE ELEMENTS; GENE-EXPRESSION; BITHORAX COMPLEX; CHROMATIN; DOMAINS; GENOME; SEGMENTATION; ORGANIZATION; COMPACTION; TRITHORAX;
D O I
10.1038/s41467-018-05945-4
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Polycomb-group proteins are conserved chromatin factors that maintain the silencing of key developmental genes, notably the Hox gene clusters, outside of their expression domains. Depletion of Polycomb repressive complex 1 (PRC1) proteins typically results in chromatin unfolding, as well as ectopic transcription. To disentangle these two phenomena, here we analyze the temporal function of two PRC1 proteins, Polyhomeotic (Ph) and Polycomb (Pc), on Hox gene clusters during Drosophila embryogenesis. We show that the absence of Ph or Pc affects the higher-order chromatin folding of Hox clusters prior to ectopic Hox gene transcription, demonstrating that PRC1 primary function during early embryogenesis is to compact its target chromatin. Moreover, the differential effects of Ph and Pc on Hox cluster folding match the differences in ectopic Hox gene expression observed in these two mutants. Our data suggest that PRC1 maintains gene silencing by folding chromatin domains and impose architectural layer to gene regulation.
引用
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页数:11
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