Blowfly olfactory biosensor's sensitivity and specificity

被引:14
作者
Huotari, M
Mela, M
机构
[1] University of Oulu, Department of Physical Sciences, Division of Biophysics
关键词
biological sensor; olfactory biosensor; extracellular recording; odour stimulation; biogenic amines; time interval to voltage conversion;
D O I
10.1016/S0925-4005(97)80003-2
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
In this article we introduce an insect (blowfly Calliphora vicina) olfactory biosensor. This biosensor has proved sensitive to the vapour of biogenic amines, and especially to 1,4-diaminobutane, According to our electrophysiological experiments the blowfly olfactory biosensor was sensitive to the 1,4-diaminobutane odour (<1 ng in stimulus air pulse), but less sensitive to 1,3-diaminopropane, 1,5-diaminopentane, 1,6-diaminohexane, 1,7-diaminoheptane and ammoniac odour (<100 mu g on stimulus cartridge). Based on time to voltage conversion and visual inspection we have determined the action potential rate as the response value. Also according to our behavioural tests the odour of 1,4-diaminobutane attracted clearly the blowflies.
引用
收藏
页码:240 / 244
页数:5
相关论文
共 4 条
  • [1] BELLI SL, 1986, ANAL LETT, V19, P403
  • [2] HUOTARI M, 1993, 9 NORD M MED BIOL EN
  • [3] RECEPTORS FOR MEAT-ODOR AND FLOWER-ODOR ON ANTENNA OF BLOWFLY CALLIPHORA-VICINA
    KAIB, M
    [J]. JOURNAL OF COMPARATIVE PHYSIOLOGY, 1974, 95 (02): : 105 - 121
  • [4] KAISSLING KE, 1987, WRIGHT LECT INSECT O