Thymosin β4 induces the expression of vascular endothelial growth factor (VEGF) in a hypoxia-inducible factor (HIF)-1α-dependent manner

被引：44
|
作者：
Jo, Jin-Ok [1 ]
Kim, Su-Ryun [3 ]
Bae, Moon-Kyung [3 ]
Kang, Yun-Jeong [1 ]
Ock, Mee Sun [1 ]
Kleinman, Hynda K. [4 ]
Cha, Hee-Jae [1 ,2 ]
机构：
[1] Kosin Univ, Dept Parasitol & Genet, Coll Med, Pusan 602703, South Korea
[2] Kosin Univ, Inst Med Sci, Coll Med, Pusan 602703, South Korea
[3] Pusan Natl Univ, Dept Physiol, Sch Dent, Pusan, South Korea
[4] Natl Inst Dent & Craniofacial Res, Craniofacial Dev Biol & Regenerat Branch, NIH, Bethesda, MD USA
来源：
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH | 2010年 / 1803卷 / 11期
关键词：
Thymosin beta 4; VEGF; HIF-1; alpha; Hypoxia; Angiogenesis; Protein stability; ACTIN-SEQUESTERING PROTEIN; FACTOR; 1-ALPHA; HAIR-GROWTH; BETA(4); GENE; METASTASIS; ACTIVATION; CELLS; STABILIZATION; CARCINOMA;
D O I：
10.1016/j.bbamcr.2010.07.005
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Thymosin beta 4 has multi-functional roles in cell physiology, but little is known about its mechanism(s) of action. We previously reported that thymosin beta 4 stimulated angiogenesis through the induction of vascular endothelial growth factor (VEGF). To identify the mechanism of VEGF induction by thymosin beta 4, we have used a luciferase assay system with VEGF in the 5' promoter region. We also analyzed the effect of thymosin beta 4 on VEGF mRNA stability and on the expression and stability of hypoxia-inducible factor (HIF)-1 alpha. We found that thymosin beta 4 induces VEGF expression by an increase in the stability of HIF-1 alpha protein. Analysis of the expression patterns of thymosin beta 4 and HIF-1 alpha in colon cancer tissue microarray showed that thymosin beta 4 and HIF-1 alpha co-localized in these biopsies. These data show that thymosin beta 4 induces the expression of VEGF indirectly by increasing the protein stability of HIF-1 alpha. (C) 2010 Elsevier B.V. All rights reserved.
引用
收藏
页码：1244 / 1251
页数：8
相关论文
共 50 条
  • [1] Hypoxia-Inducible Factor (HIF)/Vascular Endothelial Growth Factor (VEGF) Signaling in the Retina
    Kurihara, Toshihide
    Westenskow, Peter D.
    Friedlander, Martin
    RETINAL DEGENERATIVE DISEASES: MECHANISMS AND EXPERIMENTAL THERAPY, 2014, 801 : 275 - 281
  • [2] Elevated Expression of Thymosin β4, Vascular Endothelial Growth Factor (VEGF), and Hypoxia Inducible Factor (HIF)-1α in Early-Stage Cervical Cancers
    Kim, Nam Soo
    Kang, Yun-Jeong
    Jo, Jin-Ok
    Kim, Heung Yeol
    Oh, Young Rim
    Kim, Young-Ok
    Jung, Min Hyung
    Ock, Mee Sun
    Cha, Hee-Jae
    PATHOLOGY & ONCOLOGY RESEARCH, 2011, 17 (03) : 493 - 502
  • [3] Expression of Hypoxia-Inducible Factor (HIF)-1a-Vascular Endothelial Growth Factor (VEGF)-Inhibitory Growth Factor (ING)-4- axis in sarcoidosis patients
    Tzouvelekis A.
    Ntolios P.
    Karameris A.
    Koutsopoulos A.
    Boglou P.
    Koulelidis A.
    Archontogeorgis K.
    Zacharis G.
    Drakopanagiotakis F.
    Steiropoulos P.
    Anevlavis S.
    Polychronopoulos V.
    Mikroulis D.
    Bouros D.
    BMC Research Notes, 5 (1)
  • [4] Hypoxia induces autophagy in human vascular endothelial cells in a hypoxia-inducible factor 1-dependent manner
    Wu, Jianbo
    Lei, Zhen
    Yu, Jingui
    MOLECULAR MEDICINE REPORTS, 2015, 11 (04) : 2677 - 2682
  • [5] Specific inhibition of hypoxia-inducible factor (HIF)-1α activation and of vascular endothelial growth factor (VEGF) production by flavonoids
    Hasebe, Y
    Egawa, K
    Yamazaki, Y
    Kunimoto, S
    Hirai, Y
    Ida, Y
    Nose, K
    BIOLOGICAL & PHARMACEUTICAL BULLETIN, 2003, 26 (10) : 1379 - 1383
  • [6] Expression of vascular endothelial growth factor and hypoxia-inducible factor 1α in the myocardium
    Takahashi, T
    Sugishita, Y
    Shimizu, T
    Yao, A
    Harada, K
    Nagai, R
    SIGNAL TRANSDUCTION AND CARDIAC HYPERTROPHY, 2003, 7 : 439 - 447
  • [7] Estrogen induces recruitment of hypoxia-inducible factor 1β (HIF-1β) to the proximal promoter of the vascular endothelial growth factor (VEGF) gene.
    Kazi, AA
    Koos, RD
    BIOLOGY OF REPRODUCTION, 2005, : 183 - 184
  • [8] Expression and regulation of hypoxia-inducible factor 1 alpha (HIF-1 alpha) and vascular endothelial growth factor (VEGF) in systemic sclerosis.
    Distler, O
    Scheid, A
    Kowal-Bielecka, O
    Schedel, J
    Rethage, J
    Carossino, A
    Matucci-Cerinic, M
    Gay, RE
    Muller-Lidner, U
    Wenger, RH
    Gassmann, M
    Gay, S
    ARTHRITIS AND RHEUMATISM, 1999, 42 (09): : S203 - S203
  • [9] Expression of hypoxia-inducible factor 1α and vascular endothelial growth factor in pituitary adenomas
    Kyongsong Kim
    Daizo Yoshida
    Akira Teramoto
    Endocrine Pathology, 2005, 16 : 115 - 121
  • [10] Induction of hypoxia-inducible factor 1α gene expression by vascular endothelial growth factor
    Deudero, Juan Jose P.
    Caramelo, Carlos
    Castellanos, Maria Carmen
    Neria, Fernando
    Fernandez-Sanchez, Ruth
    Calabia, Olalla
    Penate, Silvia
    Gonzalez-Pacheco, Francisco Roman
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2008, 283 (17) : 11435 - 11444
12345