Endophytic colonization of plants by the biocontrol agent Rhizobium etli G12 in relation to Meloidogyne incognita infection.

被引:91
作者
Hallmann, J
Quadt-Hallmann, A
Miller, WG
Sikora, RA
Lindow, SE
机构
[1] Inst Pflanzenkrankheiten Phytomed Bodenokosyst, D-53115 Bonn, Germany
[2] USDA ARS, Food Safety & Hlth Unit, Albany, CA 94710 USA
[3] Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA
关键词
D O I
10.1094/PHYTO.2001.91.4.415
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The external and internal colonization of potato and Arabidopsis roots by the biocontrol strain Rhizobium etli G12 containing a plasmidborne trp promoter green fluorescent protein transcriptional fusion, pGT-trp, was studied in the presence and absence of the root-knot nematode Meloidogyne incognita. Plant colonization behavior and biocontrol potential of the marked strain G12(pGT-trp) was not altered compared with the parental strain. Plasmid pGT-trp was stable for more than 80 generations without selection and conferred sufficient fluorescence to detect single bacterial cells in planta. Although bacteria were found over the entire rhizoplane, they preferentially colonized root tips, the emerging lateral roots, and galled tissue caused by Meloidogyne infestation. Internal colonization of potato roots was mainly observed in epidermal cells, especially root hairs. G12(pGT-trp) colonization was also observed in inner Arabidopsis root tissues in areas of vascularization. In the presence of M. incognita, G12(pGT-trp) colonized the interior of nematode galls in high numbers. In some cases, bacterial colonization even extended from the galled tissue into adjacent root tissue. The internally colonized sites in roots were often discontinuous. Fluorescence microscopy of gfp-tagged rhizobacteria was a sensitive and a rapid technique to study external and internal colonization of plant roots by bacteria interacting with nematodes.
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页码:415 / 422
页数:8
相关论文
共 25 条
[1]   IN-SITU LOCALIZATION OF AZOSPIRILLUM-BRASILENSE IN THE RHIZOSPHERE OF WHEAT WITH FLUORESCENTLY LABELED, RIBOSOMAL-RNA-TARGETED OLIGONUCLEOTIDE PROBES AND SCANNING CONFOCAL LASER MICROSCOPY [J].
ASSMUS, B ;
HUTZLER, P ;
KIRCHHOF, G ;
AMANN, R ;
LAWRENCE, JR ;
HARTMANN, A .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1995, 61 (03) :1013-1019
[2]   Green fluorescent protein as a marker for Pseudomonas spp. [J].
Bloemberg, GV ;
OToole, GA ;
Lugtenberg, BJJ ;
Kolter, R .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1997, 63 (11) :4543-4551
[3]   FACS-optimized mutants of the green fluorescent protein (GFP) [J].
Cormack, BP ;
Valdivia, RH ;
Falkow, S .
GENE, 1996, 173 (01) :33-38
[4]   INVOLVEMENT OF BACTERIAL POLYSACCHARIDES IN PLANT PATHOGENESIS [J].
DENNY, TP .
ANNUAL REVIEW OF PHYTOPATHOLOGY, 1995, 33 :173-197
[5]   BROAD HOST RANGE DNA CLONING SYSTEM FOR GRAM-NEGATIVE BACTERIA - CONSTRUCTION OF A GENE BANK OF RHIZOBIUM-MELILOTI [J].
DITTA, G ;
STANFIELD, S ;
CORBIN, D ;
HELINSKI, DR .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1980, 77 (12) :7347-7351
[6]   Use of green fluorescent protein to detect expression of nif genes of Azoarcus sp. BH72, a grass-associated diazotroph, on rice roots [J].
Egener, T ;
Hurek, T ;
Reinhold-Hurek, B .
MOLECULAR PLANT-MICROBE INTERACTIONS, 1998, 11 (01) :71-75
[7]  
ELLIOTT LF, 1984, AM SOC AGRON SPEC PU, V47, P1
[8]   Use of green fluorescent protein to visualize the early events of symbiosis between Rhizobium meliloti and alfalfa (Medicago sativa) [J].
Gage, DJ ;
Bobo, T ;
Long, SR .
JOURNAL OF BACTERIOLOGY, 1996, 178 (24) :7159-7166
[9]   Chitin-mediated changes in bacterial communities of the soil, rhizosphere and within roots of cotton in relation to nematode control [J].
Hallmann, J ;
Rodríguez-Kábana, R ;
Kloepper, JW .
SOIL BIOLOGY & BIOCHEMISTRY, 1999, 31 (04) :551-560
[10]   Bacterial endophytes in agricultural crops [J].
Hallmann, J ;
QuadtHallmann, A ;
Mahaffee, WF ;
Kloepper, JW .
CANADIAN JOURNAL OF MICROBIOLOGY, 1997, 43 (10) :895-914