Assessing corneal biomechanics with Brillouin spectro-microscopy

被引：49

作者：

Lepert, Guillaume ^{[1
]}

Gouveia, Ricardo M. ^{[2
]}

Connon, Che J. ^{[2
]}

Paterson, Carl ^{[1
]}

机构：

[1] Univ London Imperial Coll Sci Technol & Med, Blackett Lab, London SW7 2BW, England

[2] Newcastle Univ, Inst Genet Med, Int Ctr Life, Cent Pkwy, Newcastle Upon Tyne NE1 3BZ, Tyne & Wear, England

来源：

FARADAY DISCUSSIONS | 2016年 / 187卷

基金：

英国生物技术与生命科学研究理事会; 英国医学研究理事会;

关键词：

OPTICAL MICROSCOPY; EX-VIVO; SCATTERING; PERFORMANCE; CELLS;

D O I：

10.1039/c5fd00152h

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

A new Brillouin spectro-microscope was designed and built to investigate the mechanical properties of bovine and human corneas. This instrument integrates a single-stage virtually imaged phased array spectrometer with a novel adaptive-optics interferometric filter to achieve unprecedented rejection of the elastic background signal. As a result, highly-resolved, reproducible data from both thin and thick collagen-based materials were obtained. In particular, this technique is capable of rigorously measuring the relative stiffness of different areas of human corneas, thus providing a true non-contact method to characterise the fundamental mechanical features of both live and fixed biological tissue samples.

引用

页码：415 / 428

页数：14

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