Hydrophoretic high-throughput selection of platelets in physiological shear-stress range

被引：68

作者：

Choi, Sungyoung ^{[1
]}

Ku, Taeyun ^{[2
]}

Song, Seungjeong ^{[1
]}

Choi, Chulhee ^{[1
,2
]}

Park, Je-Kyun ^{[1
,3
]}

机构：

[1] Korea Adv Inst Sci & Technol, Coll Life Sci & Bioengn, Dept Bio & Brain Engn, Taejon 305701, South Korea

[2] Korea Adv Inst Sci & Technol, Grad Sch Med Sci & Engn, Taejon 305701, South Korea

[3] Korea Adv Inst Sci & Technol, Inst NanoCentury, Taejon 305701, South Korea

来源：

LAB ON A CHIP | 2011年 / 11卷 / 03期

关键词：

RICH PLASMA; CELL-SEPARATION; WHOLE-BLOOD; FILTRATION; ACTIVATION; PARTICLE;

D O I：

10.1039/c0lc00148a

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A gentle, but fast means for low-stress, high-throughput platelet purification is of significant clinical and biotechnological utility. Current implementations to sort platelets, however, require an external physical field, specialized buffer, or the harsh separation condition of high shear stress that tends to cause platelet stimulation. Here we report the use of hydrophoretic size separation in a wider channel and its parallelization to augment its throughput capability, maintaining physiological shear-stress range. We demonstrate a parallelized device comprising 10 stacks of the wide-channel hydrophoresis device, yielding a throughput of 2.9 million cells s(-1) and a platelet purity of 76.8%. The use of the wide channel for hydrophoresis also facilitates clogging-free separation by sorting blood clots and plaques. The wide-channel hydrophoresis offers the potential for gentle, fast, clogging-free sorting of rare blood cells with extreme throughput capabilities.

引用

页码：413 / 418

页数：6

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