Vascular Tissue Engineering: Effects of Integrating Collagen into a PCL Based Nanofiber Material

被引：45

作者：

Bertram, Ulf ^{[1
]}

Steiner, Dominik ^{[1
]}

Poppitz, Benjamin ^{[2
]}

Dippold, Dirk ^{[2
]}

Koehn, Katrin ^{[1
]}

Beier, Justus P. ^{[1
]}

Detsch, Rainer ^{[3
]}

Boccaccini, Aldo R. ^{[3
]}

Schubert, Dirk W. ^{[2
]}

Horch, Raymund E. ^{[1
]}

Arkudas, Andreas ^{[1
]}

机构：

[1] Friedrich Alexander Univ Erlangen Nurnberg FAU, Dept Plast & Hand Surg, Univ Hosp Erlangen, Erlangen, Germany

[2] Friedrich Alexander Univ Erlangen Nurnberg FAU, Inst Polymer Mat, Erlangen, Germany

[3] Friedrich Alexander Univ Erlangen Nurnberg FAU, Dept Mat Sci & Engn, Inst Biomat, Erlangen, Germany

来源：

BIOMED RESEARCH INTERNATIONAL | 2017年 / 2017卷

关键词：

ENDOTHELIAL PROGENITOR CELLS; MORPHOLOGY; PROLIFERATION; POLY(EPSILON-CAPROLACTONE); MORPHOGENESIS; ORGANIZATION; EXPRESSION; SCAFFOLD;

D O I：

10.1155/2017/9616939

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The engineering of vascular grafts is a growing field in regenerative medicine. Although numerous attempts have been made, the current vascular grafts made of polyurethane (PU), Dacron (R), or Teflon (R) still display unsatisfying results. Electrospinning of biopolymers and native proteins has been in the focus of research to imitate the extracellular matrix (ECM) of vessels to produce a small caliber, off-the-shelf tissue engineered vascular graft (TEVG) as a substitute for poorly performing PU, Dacron, or Teflon prostheses. Blended poly-epsilon-caprolactone (PCL)/collagen grafts have shown promising results regarding biomechanical and cell supporting features. In order to find a suitable PCL/collagen blend, we fabricated plane electrospun PCL scaffolds using various collagen type I concentrations ranging from 5% to 75%. We analyzed biocompatibility and morphological aspects in vitro. Our results show beneficial features of collagen I integration regarding cell viability and functionality, but also adverse effects like the loss of a confluent monolayer at high concentrations of collagen. Furthermore, electrospun PCL scaffolds containing 25% collagen I seem to be ideal for engineering vascular grafts.

引用

页数：11

共 43 条

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