Coomassie blue is sufficient for specific protein detection of aptamer-conjugated chips

被引：14
作者：
Lee, Gwan-Ho
Hah, Sang Soo [1 ]
机构：
[1] Kyung Hee Univ, Dept Chem, Res Inst Basic Sci, Seoul 130701, South Korea
来源：
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2012年 / 22卷 / 04期
基金：
新加坡国家研究基金会;
关键词：
Aptamer; Biochip; Protein quantitation; Coomassie blue; DNA; NANOPARTICLES;
D O I：
10.1016/j.bmcl.2012.01.012
中图分类号：
R914 [药物化学];
学科分类号：
100701 ;
摘要：
An aptamer-based biochip for protein detection and quantitation which combines the recent biochip technology and the conventional staining methods, is described. Using a model system comprising His-tagged proteins as the analyte and single-stranded RNA aptamers specific for His-tagged proteins as immobilized ligands on chips, we could demonstrate that aptamers were equivalent or superior to antibodies in terms of specificity and sensitivity, respectively. The sensor has the characteristics of good stability, reproducibility and reusability, with detection limit as low as 85 ng/mL His-tagged protein. It has been demonstrated that the sensor can be stored for at least 4 weeks and reused with reasonable reduction rate of staining intensity. In conclusion, we could show the suitability of nucleic acid aptamers as low molecular weight receptors on biochips for sensitive and specific protein detection and quantitation. (C) 2012 Elsevier Ltd. All rights reserved.
引用
收藏
页码：1520 / 1522
页数：3
相关论文
共 16 条
[1]   Sensing capability of the localized surface plasmon resonance of gold nanorods [J].
Chen, Cheng-Dah ;
Cheng, Shu-Fang ;
Chau, Lai-Kwan ;
Wang, C. R. Chris .
BIOSENSORS & BIOELECTRONICS, 2007, 22 (06) :926-932
[2]   Aptamers Generated from Cell-SELEX for Molecular Medicine: A Chemical Biology Approach [J].
Fang, Xiaohong ;
Tan, Weihong .
ACCOUNTS OF CHEMICAL RESEARCH, 2010, 43 (01) :48-57
[3]   Fluorescent amplifying recognition for DNA G-quadruplex folding with a cationic conjugated polymer: A platform for homogeneous potassium detection [J].
He, F ;
Tang, YL ;
Wang, S ;
Li, YL ;
Zhu, DB .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2005, 127 (35) :12343-12346
[4]   Aptamer in Bioanalytical Applications [J].
Iliuk, Anton B. ;
Hu, Lianghai ;
Tao, W. Andy .
ANALYTICAL CHEMISTRY, 2011, 83 (12) :4440-4452
[5]   Two-step synthesis of 5′-deoxy-5′-thioguanosine-5′-monophosphorothioate and its incorporation efficiency into 5′-terminus of RNA for preparation of thiol-functionalized RNA [J].
Kim, Il-Hyun ;
Shin, Seonmi ;
Jeong, Yong-Joo ;
Hah, Sang Soo .
TETRAHEDRON LETTERS, 2010, 51 (26) :3446-3448
[6]   Binding of uranyl ion by a DNA aptamer attached to a solid support [J].
Kim, Jisu ;
Kim, Min Young ;
Kim, Hoon Sik ;
Hah, Sang Soo .
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 2011, 21 (13) :4020-4022
[7]   Fast colorimetric sensing of adenosine and cocaine based on a general sensor design involving aptamers and nanoparticles [J].
Liu, JW ;
Lu, Y .
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2006, 45 (01) :90-94
[8]   Aptamers: An emerging class of therapeutics [J].
Nimjee, SM ;
Rusconi, CP ;
Sullenger, BA .
ANNUAL REVIEW OF MEDICINE, 2005, 56 :555-+
[9]   Aptamer-functionalized Au nanoparticles for the amplified optical detection of thrombin [J].
Pavlov, V ;
Xiao, Y ;
Shlyahovsky, B ;
Willner, I .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (38) :11768-11769
[10]   Abasic-site-containing oligodeoxynucleotides as aptamers for riboflavin [J].
Sankaran, NB ;
Nishizawa, S ;
Seino, T ;
Yoshimoto, K ;
Teramae, N .
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2006, 45 (10) :1563-1568
12