Isolation and characterization of a mutant Chinese hamster ovary cell line that is resistant to Chlamydia trachomatis infection at a novel step in the attachment process

被引：46

作者：

Carabeo, RA ^{[1
]}

Hackstadt, T ^{[1
]}

机构：

[1] NIAID, Host Parasite Interact Sect, Intracellular Parasites Lab, Rocky Mt Labs,NIH, Hamilton, MT 59840 USA

来源：

INFECTION AND IMMUNITY | 2001年 / 69卷 / 09期

关键词：

D O I：

10.1128/IAI.69.9.5899-5904.2001

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Host factors involved in Chlamydia trachomatis pathogenesis were investigated by random chemical mu. tagenesis of Chinese hamster ovary (CHO-KI) cells followed by selection for clones resistant to chlamydial infection. A clonal mutant cell line, D4.1-3, refractory to infection by the C. trachomatis L2 serovar was isolated. The D4.1-3 cell line appears to be lacking in a previously undescribed temperature-dependent and heparin-resistant binding step that occurs subsequent to engagement of cell surface heparan sulfate by L2 elementary bodies. This novel binding step differentiates the lymphogranuloma venereum (LGV) serovar from other serovars and may contribute the different pathologies associated with LGV and non-LGV strains.

引用

页码：5899 / 5904

页数：6

共 30 条

[1] Isolation and characterization of a Chinese hamster ovary mutant cell line with altered sensitivity to vaccinia virus killing [J].