RD Antigen Based Nanovaccine Imparts Long Term Protection by Inducing Memory Response against Experimental Murine Tuberculosis

被引：34

作者：

Ansari, Mairaj Ahmed ^{[1
]}

Zubair, Swaleha ^{[2
]}

Mahmood, Anjum ^{[3
]}

Gupta, Pushpa ^{[4
]}

Khan, Aijaz A. ^{[5
]}

Gupta, Umesh D. ^{[4
]}

Arora, Ashish ^{[3
]}

Owais, Mohammad ^{[1
]}

机构：

[1] Aligarh Muslim Univ, Interdisciplinary Biotechnol Unit, Aligarh, Uttar Pradesh, India

[2] Aligarh Muslim Univ, Womens Coll, Aligarh, Uttar Pradesh, India

[3] CDRI, Mol & Struct Biol Div, Lucknow, Uttar Pradesh, India

[4] Natl JALMA Inst Leprosy & OMD, Agra, Uttar Pradesh, India

[5] JNMC, Dept Anat, Aligarh, Uttar Pradesh, India

来源：

PLOS ONE | 2011年 / 6卷 / 08期

关键词：

ANTITUBERCULOSIS DRUG-RESISTANCE; VACCINES; SUBUNIT; ARCHAEOSOMES; DELIVERY; PROTEIN; BCG;

D O I：

10.1371/journal.pone.0022889

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Background: The absence of certain genomic loci that are present in most of the virulent strains of Mycobacterium tuberculosis as well as lack of lasting memory responses are some of the major causes attributed to the non effectiveness of Bacille Calmette-Gue'rin (BCG) vaccine. Immunization schedules addressing these issues can offer better strategy for protection against tuberculosis. Methods: The immunological responses evoked upon administration of archaeosome based antigen delivery system comprising T cell antigen, Rv3619c (an ESAT-6 family protein), has been assessed against experimental murine tuberculosis in BALB/c mice. Results: Archaeosome based subunit vaccine has been found to elicit type-1 cytokines in the immunized mice. Besides effective T cell memory response, the Rv3619c based vaccine was able to reduce mycobacterial burden in the animals challenged with Mycobacterium tuberculosis infection. Conclusion: The data of the present study suggest that archaeosome encapsulated RD gene products offer substantial protection against M. tuberculosis infection.

引用

页数：12

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