Leptin and Its Signaling Are Not Involved in Zebrafish Puberty Onset

被引:12
|
作者
Hu, Zhe [1 ,2 ]
Ai, Nana [1 ,2 ]
Chen, Weiting [1 ,2 ]
Wong, Queenie Wing-Lei [1 ,2 ]
Ge, Wei [1 ,2 ]
机构
[1] Univ Macau, Fac Hlth Sci, Dept Biomed Sci, Taipa 999078, Macao, Peoples R China
[2] Univ Macau, Fac Hlth Sci, Ctr Reprod Dev Aging CRDA, Taipa 999078, Macao, Peoples R China
关键词
FOOD-INTAKE; GLUCOSE-HOMEOSTASIS; MISSENSE MUTATION; SEXUAL-MATURATION; BODY-WEIGHT; FEMALE MICE; GENE; OBESE; EXPRESSION; GROWTH;
D O I
10.1093/biolre/ioac005
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Leptin is a peptide hormone secreted from the adipose tissues and its signaling plays a central role in metabolic regulation of growth, especially on fat mass. In addition, leptin is also involved in regulating reproduction in mammals. In teleosts, there are two leptin ligands (lepa and lepb) and one cognate leptin receptor (lepr); however, their functions are still elusive. In this study, we created null-function mutants for lepa, lepb and lepr in zebrafish using CRISPR/Cas9 method and analyzed their phenotypes with emphasis on puberty onset, one major function widely reported for leptin in mammals. We demonstrated that the loss of leptin ligands or their receptor resulted in no obesity from prepubertal stage to adulthood. We then focused on leptin involvement in controlling puberty onset. We first confirmed the somatic threshold for puberty onset in females and proposed a criterion and somatic threshold for male puberty onset. We examined gonadal development and sex maturation in different genotypic combinations including single mutants (lepa(-/-), lepb(-/-) and lepr(-/-)), double mutants (lepa(-/-);lepb(-/-)) and triple mutants (lepa(-/-);lepb(-/-);lepr(-/-)). Our results showed that once the fish reached the thresholds, the siblings of all genotypes displayed comparable gonadal development in both sexes without obvious signs of changed puberty onset. In conclusion, this comprehensive genetic study on the lep-lepr system demonstrated that in contrast to its counterpart in mammals, leptin system plays little role in controlling growth and reproduction especially puberty onset in zebrafish.
引用
收藏
页码:928 / 942
页数:15
相关论文
共 50 条
  • [21] Leptin and puberty
    Clayton, PE
    Trueman, JA
    ARCHIVES OF DISEASE IN CHILDHOOD, 2000, 83 (01) : 1 - 3
  • [22] Impaired leptin signaling causes subfertility in female zebrafish
    Tsakoumis, Emmanouil
    Ahi, Ehsan Pashay
    Schmitz, Monika
    MOLECULAR AND CELLULAR ENDOCRINOLOGY, 2022, 546
  • [23] Leptin in puberty
    Apter, D
    CLINICAL ENDOCRINOLOGY, 1997, 47 (02) : 175 - 176
  • [24] Leptin and puberty
    Urbanski, HF
    TRENDS IN ENDOCRINOLOGY AND METABOLISM, 2001, 12 (10): : 428 - 429
  • [25] A reassessment of leptin's role in triggering the onset of puberty in the rat and mouse
    Cheung, CC
    Thornton, JE
    Nurani, SD
    Clifton, DK
    Steiner, RA
    NEUROENDOCRINOLOGY, 2001, 74 (01) : 12 - 21
  • [26] Leptin attenuates follicular apoptosis and accelerates the onset of puberty in immature rats
    Almog, B
    Gold, R
    Tajima, K
    Dantes, A
    Salim, K
    Rubinstein, M
    Barkan, D
    Homburg, R
    Lessing, JB
    Nevo, N
    Gertler, A
    Amsterdam, A
    MOLECULAR AND CELLULAR ENDOCRINOLOGY, 2001, 183 (1-2) : 179 - 191
  • [27] Leptin and puberty
    Rogol, AD
    JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 1998, 83 (04): : 1089 - 1090
  • [28] Astrocyte-neuron signaling and the onset of female puberty
    Ojeda, SR
    Ma, YJ
    Dziedzic, B
    Prevot, V
    ONSET OF PUBERTY IN PERSPECTIVE, 2000, 1202 : 41 - 57
  • [29] Zebrafish nma is involved in TGFβ family signaling
    Tsang, M
    Kim, R
    de Caestecker, MP
    Kudoh, T
    Roberts, AB
    Dawid, IB
    GENESIS, 2000, 28 (02) : 47 - 57
  • [30] The Onset of Puberty and its Impact on Activity Levels
    Jeffreys, Renee
    Pinney, Susan M.
    Succop, Paul
    Sternfeld, Barbara
    Teitelbaum, Susan L.
    Galvez, Maida P.
    Wolff, Mary S.
    Kushi, Lawrence H.
    Biro, Frank M.
    MEDICINE AND SCIENCE IN SPORTS AND EXERCISE, 2014, 46 (05): : 781 - 781