CHAMPURU 1.0: a computer software for unraveling mixtures of two DNA sequences of unequal lengths

被引：99

作者：

Flot, Jean-Francois ^{[1
]}

机构：

[1] Museum Natl Hist Nat, UMR UPMC CNRS MNHN IRD 7138, Dept Systemat & Evolut, F-75231 Paris 05, France

来源：

MOLECULAR ECOLOGY NOTES | 2007年 / 7卷 / 06期

关键词：

double peaks; haplotyping; indels; length variant heterozygote; mixed trace; sequencing;

D O I：

10.1111/j.1471-8286.2007.01857.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

CHAMPURU is an interactive, user-friendly web software that facilitates the deconvolution of mixed chromatograms obtained when sequencing directly mixtures of two DNA templates of unequal lengths. The program takes as input two strings of characters describing the forward and reverse chromatograms as obtained by direct sequencing and returns, most often after several iterations aimed at correcting basecalling errors, the sequences of the two templates present in the mixture.

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收藏

页码：974 / 977

页数：4

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