Recent advances in site-directed spin labeling of proteins

被引：483

作者：

Hubbell, WL ^{[1
]}

Gross, A

Langen, R

Lietzow, MA

机构：

[1] Univ Calif Los Angeles, Jules Stein Eye Inst, Los Angeles, CA 90095 USA

[2] Univ Calif Los Angeles, Dept Chem & Biochem, Los Angeles, CA 90095 USA

来源：

CURRENT OPINION IN STRUCTURAL BIOLOGY | 1998年 / 8卷 / 05期

关键词：

D O I：

10.1016/S0959-440X(98)80158-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Site-directed spin labeling of proteins is experiencing a phase of rapid technical evolution, application and evaluation, New strategies have been introduced for determining membrane protein topography, electrostatic potentials, the orientation of proteins at membrane surfaces and inter-residue distances, New applications include studies of beta strands, structure mapping using spin-spin interactions, domain motions in soluble proteins and extensive structural analysis of a number of membrane and soluble proteins.

引用

页码：649 / 656

页数：8

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