Cdc42 activates paracellular transport in polarised submandibular gland cells

被引：1

作者：

Okabayashi, Ken ^{[1
]}

Nakamura, Mari ^{[1
,2
,3
]}

Narita, Takanori ^{[2
]}

机构：

[1] Nihon Univ, Coll Bioresource Sci, Dept Vet Med, Lab Vet Biochem, Fujisawa, Kanagawa, Japan

[2] Nihon Univ, Coll Bioresource Sci, Dept Vet Med, Lab Mol Biol, 1866 Kameino, Fujisawa, Kanagawa 2520880, Japan

[3] Tokyo Metropolitan Inst Publ Hlth, Dept Pharmaceut & Environm Sci, Shinjuku Ku, Tokyo, Japan

来源：

ARCHIVES OF ORAL BIOLOGY | 2021年 / 132卷

关键词：

Cdc42; Paracellular transport; Saliva; Submandibular gland; BARRIER FUNCTION; EXPRESSION; GTPASES; RHO;

D O I：

10.1016/j.archoralbio.2021.105276

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

Objective: The physiological expression of cell division cycle 42 (cdc42) in major salivary glands, and paracellular transport of fluorescein isothiocyanate-dextran (FITC-dextran) in SMIE cells, which regulate cdc42 expression, was investigated to clarify the involvement of cdc42 in salivary production. Design: The physiological expression of cdc42 in the rat submandibular gland, parotid gland, sublingual gland, and SMIE cells was detected using SDS-PAGE and western blotting. The paracellular transport of FITC-dextran in transwells was compared in transfected SMIE cells, exhibiting up- or downregulated cdc42 expression. Results: Cdc42 was expressed in all major salivary glands and SMIE cells. SMIE cells transfected with the cdc42 plasmid had an increase efflux. In addition, SMIE cells transfected with the cdc42 siRNA showed decreased efflux. Conclusion: We suggest that cdc42 enhances paracellular transport in salivary glands without any morphological changes, including cell-cell adhesion.

引用

页数：6

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