Electroosmotic flow dynamics during a field-amplified sample stacking experiment have been studied experimentally using the periodic photobleaching of a dilute, neutral fluorophore added to the separation buffer. The effects of hydrodynamically injecting different sample plug lengths containing a mixture of arsenic compounds dissolved in 0.125 mM (120, 240, and 600 s) and 41.7 muM (27, 45, and 74 s) phosphate buffer with a separation buffer concentration of 12.5 mM phosphate buffer were examined. Changes in electroosmotic flow during sample stacking and separation were monitored at a rate of 1 Hz. The observed effects of increasing the sample plug length on electroosmotic flow and electrophoretic current agreed qualitatively with predictions by theoretical models presented in the literature. Electroosmotic flow changes on the order of 100% (1.6-3.3 mm/s) were observed. Broadening of the flow monitoring peaks has been used to examine parabolic flow due to the discontinuous buffer systems used for sample stacking.
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Univ Tasmania, Sch Phys Sci, Australian Ctr Res Separat Sci, Hobart, Tas 7001, AustraliaUniv Tasmania, Sch Phys Sci, Australian Ctr Res Separat Sci, Hobart, Tas 7001, Australia
Gstoettenmayr, Daniel
Quirino, Joselito
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Univ Tasmania, Sch Phys Sci, Australian Ctr Res Separat Sci, Hobart, Tas 7001, AustraliaUniv Tasmania, Sch Phys Sci, Australian Ctr Res Separat Sci, Hobart, Tas 7001, Australia
Quirino, Joselito
Ivory, Cornelius F.
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Washington State Univ, Voiland Sch Chem Engn & Bioengn, Pullman, WA 99164 USAUniv Tasmania, Sch Phys Sci, Australian Ctr Res Separat Sci, Hobart, Tas 7001, Australia
Ivory, Cornelius F.
Breadmore, Michael
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Univ Tasmania, Sch Phys Sci, Australian Ctr Res Separat Sci, Hobart, Tas 7001, AustraliaUniv Tasmania, Sch Phys Sci, Australian Ctr Res Separat Sci, Hobart, Tas 7001, Australia
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Kyoto Univ, Grad Sch Engn, Dept Mat Chem, Nishikyo Ku, Kyoto 6158510, JapanKyoto Univ, Grad Sch Engn, Dept Mat Chem, Nishikyo Ku, Kyoto 6158510, Japan
Kawai, Takayuki
Koino, Hiroshi
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Kyoto Univ, Grad Sch Engn, Dept Mat Chem, Nishikyo Ku, Kyoto 6158510, JapanKyoto Univ, Grad Sch Engn, Dept Mat Chem, Nishikyo Ku, Kyoto 6158510, Japan
Koino, Hiroshi
Sueyoshi, Kenji
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Kyoto Univ, Grad Sch Engn, Dept Mat Chem, Nishikyo Ku, Kyoto 6158510, JapanKyoto Univ, Grad Sch Engn, Dept Mat Chem, Nishikyo Ku, Kyoto 6158510, Japan
Sueyoshi, Kenji
Kitagawa, Fumihiko
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Kyoto Univ, Grad Sch Engn, Dept Mat Chem, Nishikyo Ku, Kyoto 6158510, JapanKyoto Univ, Grad Sch Engn, Dept Mat Chem, Nishikyo Ku, Kyoto 6158510, Japan
Kitagawa, Fumihiko
Otsuka, Koji
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Kyoto Univ, Grad Sch Engn, Dept Mat Chem, Nishikyo Ku, Kyoto 6158510, JapanKyoto Univ, Grad Sch Engn, Dept Mat Chem, Nishikyo Ku, Kyoto 6158510, Japan