Draft Genomic Resources for the Brown Rot Fungal Pathogen Monilinia laxa

被引：19

作者：

Landi, Lucia ^{[1
]}

Pollastro, Stefania ^{[2
,3
]}

Rotolo, Caterina ^{[2
]}

Romanazzi, Gianfranco ^{[1
]}

Faretra, Francesco ^{[2
,3
]}

Angelini, Rita Milvia De Miccolis ^{[2
,3
]}

机构：

[1] Marche Polytech Univ, Dept Agr Food & Environm Sci, Via Brecce Bianche 10, I-60131 Ancona, Italy

[2] Univ Bad Aldo Moro, Dept Soil Plant & Food Sci, Via G Amendola 165-A, I-70126 Bari, Italy

[3] SELGE Network, Via G Amendola 165-A, I-70126 Bari, Italy

来源：

MOLECULAR PLANT-MICROBE INTERACTIONS | 2020年 / 33卷 / 02期

关键词：

brown rot; de novo assembly; genomics; metabolomics; next-generation sequencing; proteomics; stone fruit; third-generation sequencing;

D O I：

10.1094/MPMI-08-19-0225-A

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Monilinia laxa is the causal agent of brown rot on stone fruit, and it can cause heavy yield losses during field production and postharvest storage. This article reports the draft genome assembly of the M. laxa Mlax316 strain, obtained using a hybrid genome assembly with both Illumine short-reads and PacBio long-reads sequencing technologies. The complete draft genome consists of 49 scaffolds with total size of 42.81 Mb, and scaffold N-50 of 2,449.4 kb. Annotation of the M. laxa assembly identified 11,163 genes and 12,424 proteins which were functionally annotated. This new genome draft improves current genomic resources available for M. laxa and represents a useful tool for further research into its interactions with host plants and into evolution in the Monilinia genus.

引用

页码：145 / 148

页数：4

共 23 条

[1]

Abate D, 2018, PLANT DIS, V102, P1708, DOI [10.1094/PDIS-08-17-1314-RE, 10.1094/pdis-08-17-1314-re]

[2] Mating System in the Brown Rot Pathogens Monilinia fructicola, M-laxa, and M-fructigena [J].

Abate, Domenico ;

Angelini, Rita M. De Miccolis ;

Rotolo, Caterina ;

Pollastro, Stefania ;

Faretra, Francesco .

PHYTOPATHOLOGY, 2018, 108 (11) :1315-1325

[3] De novo assembly and comparative transcriptome analysis of Monilinia fructicola, Monilinia laxa and Monilinia fructigena, the causal agents of brown rot on stone fruits [J].

Angelini, Rita M. De Miccolis ;

Abate, Domenico ;

Rotolo, Caterina ;

Gerin, Donato ;

Pollastro, Stefania ;

Faretra, Francesco .

BMC GENOMICS, 2018, 19

[4] New High-Quality Draft Genome of the Brown Rot Fungal Pathogen Monilinia fructkola [J].

Angelini, Rita Milvia De Miccolis ;

Romanazzi, Gianfranco ;

Pollastro, Stefania ;

Rotolo, Caterina ;

Faretra, Francesco ;

Landi, Lucia .

GENOME BIOLOGY AND EVOLUTION, 2019, 11 (10) :2850-2855

[5] Trimmomatic: a flexible trimmer for Illumina sequence data [J].

Bolger, Anthony M. ;

Lohse, Marc ;

Usadel, Bjoern .

BIOINFORMATICS, 2014, 30 (15) :2114-2120

[6]

Byrde R.J.V., 1977, BROWN ROT FUNGI FRUI

[7] Mapping single molecule sequencing reads using basic local alignment with successive refinement (BLASR): application and theory [J].

Chaisson, Mark J. ;

Tesler, Glenn .

BMC BIOINFORMATICS, 2012, 13

[8]

Hewitt WB, 1939, PHYTOPATHOLOGY, V29, P337

[9] Genome sequence of the brown rot fungal pathogen Monilinia fructigena [J].

Lucia Landi ;

Rita M. De Miccolis Angelini ;

Stefania Pollastro ;

Domenico Abate ;

Francesco Faretra ;

Gianfranco Romanazzi .

BMC Research Notes, 11 (1)

[10]

Martini C., 2014, Postharvest Decay Control Strategies, P233, DOI 10.1016/B978-0-12-411552-1.00007-7

← 1 2 3 →