Metabolomics reveals impact of seven functional foods on metabolic pathways in a gut microbiota model

被引:77
作者
Farag, Mohamed A. [1 ,2 ]
Abdelwareth, Amr [2 ]
Sallam, Ibrahim E. [3 ]
el Shorbagi, Mohamed [4 ]
Jehmlich, Nico [5 ]
Fritz-Wallace, Katarina [5 ]
Schaepe, Stephanie Serena [5 ]
Rolle-Kampczyk, Ulrike [5 ]
Ehrlich, Anja [6 ]
Wessjohann, Ludger A. [6 ]
von Bergen, Martin [5 ,7 ]
机构
[1] Cairo Univ, Coll Pharm, Pharmacognosy Dept, Kasr El Aini St, Cairo 11562, Egypt
[2] Amer Univ Cairo, Sch Sci & Engn, Dept Chem, New Cairo 11835, Egypt
[3] October Univ Modern Sci & Arts MSA, Coll Pharm, Pharmacognosy Dept, 6th Of October City 12566, Egypt
[4] Menouffia Univ, Chem Dept, Shibin Al Kawm, Al Minufiyah, Egypt
[5] Helmholtz Ctr Environm Res UFZ GmbH, Dept Mol Syst Biol, Leipzig, Germany
[6] Leibniz Inst Plant Biochem, Dept Bioorgan Chem, Weinberg 3, D-06120 Halle, Saale, Germany
[7] Univ Leipzig, Fac Life Sci, Inst Biochem, Talstr 33, D-04103 Leipzig, Germany
关键词
Functional foods; Gut microbiota; Metabolomics; GCMS; Chemometrics; GASTROINTESTINAL MICROBIOTA; GALLIC ACID; ANTIOXIDANT; POLYPHENOLS; PERFORMANCE; PROPIONATE; COFFEE; TIME;
D O I
10.1016/j.jare.2020.01.001
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Functional food defined as dietary supplements that in addition to their nutritional values, can beneficially modulate body functions becomes more and more popular but the reaction of the intestinal microbiota to it is largely unknown. In order to analyse the impact of functional food on the microbiota itself it is necessary to focus on the physiology of the microbiota, which can be assessed in a whole by untargeted metabolomics. Obtaining a detailed description of the gut microbiota reaction to food ingredients can be a key to understand how these organisms regulate and bioprocess many of these food components. Extracts prepared from seven chief functional foods, namely green tea, black tea, Opuntia ficus-indica (prickly pear, cactus pear), black coffee, green coffee, pomegranate, and sumac were administered to a gut consortium culture encompassing 8 microbes which are resembling, to a large extent, the metabolic activities found in the human gut. Samples were harvested at 0.5 and 24 h post addition of functional food extract and from blank culture in parallel and analysed for its metabolites composition using gas chromatography coupled to mass spectrometry detection (GC-MS). A total of 131 metabolites were identified belonging to organic acids, alcohols, amino acids, fatty acids, inorganic compounds, nitrogenous compounds, nucleic acids, phenolics, steroids and sugars, with amino acids as the most abundant class in cultures. Considering the complexity of such datasets, multivariate data analyses were employed to classify samples and investigate how functional foods influence gut microbiota metabolisms. Results from this study provided a first insights regarding how functional foods alter gut metabolism through either induction or inhibition of certain metabolic pathways, i.e. GABA production in the presence of higher acidity induced by functional food metabolites such as polyphenols. Likewise, functional food metabolites i.e., purine alkaloids acted themselves as direct substrate in microbiota metabolism. (C) 2019 The Authors. Published by Elsevier B.V. on behalf of Cairo University.
引用
收藏
页码:47 / 59
页数:13
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