Bisulfite-free analysis of 5MeC-binding proteins and locus-specific methylation density using a microparticle-based flow cytometry assay

被引：11

作者：

Corrie, Simon R. ^{[1
,2
]}

Sova, Pavel ^{[2
]}

Feng, Qinghua ^{[2
]}

Blair, Tiffany ^{[2
]}

Kiviat, Nancy B. ^{[2
]}

Trau, Matt ^{[1
]}

机构：

[1] Univ Queensland, Australian Inst Bioengn & Nanotechnol, Ctr Biomarker Res & Dev, Brisbane, Qld, Australia

[2] Univ Washington, Sch Med, Dept Pathol, Seattle, WA 98195 USA

来源：

ANALYST | 2011年 / 136卷 / 04期

关键词：

DNA METHYLATION; ORGANOSILICA MICROSPHERES; MICROARRAY ANALYSIS; LINE-1; METHYLATION; GENE-EXPRESSION; CANCER-PATIENTS; BREAST-CANCER; PLASMA DNA; CELLS; TECHNOLOGY;

D O I：

10.1039/c0an00790k

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

DNA methylation analysis is emerging as a new technique with potential capabilities for early cancer detection. However, current state-of-the-art techniques are not easily translatable into routine clinical methods. Herein we describe a bead-based flow cytometry assay which combines DNA hybridization to microparticles with 5MeC-specific proteins/antibodies. These assays can be used to study the binding properties of current and emerging 5MeC-binding proteins and may also have potential in the measurement of 5MeC density in clinical samples for cancer detection.

引用

页码：688 / 691

页数：4

共 28 条

[1] Differential methylation of human LINE-1 retrotransposons in malignant cells [J].

Alves, G ;

Tatro, A ;

Fanning, T .

GENE, 1996, 176 (1-2) :39-44

[2] LINE-1 methylation in plasma DNA as a biomarker of activity of DNA methylation inhibitors in patients with solid tumors [J].

Aparicio, Ana ;

North, Brittany ;

Barske, Lindsey ;

Wang, Xuemei ;

Bollati, Valentina ;

Weisenberger, Daniel ;

Yoo, Christine ;

Tannir, Nizar ;

Horne, Erin ;

Groshen, Susan ;

Jones, Peter ;

Yang, Allen ;

Issa, Jean-Pierre .

EPIGENETICS, 2009, 4 (03) :176-184

[3] Epigenetic gene silencing in cancer - a mechanism for early oncogenic pathway addiction? [J].

Baylin, SB ;

Ohm, JE .

NATURE REVIEWS CANCER, 2006, 6 (02) :107-116

[4] DNA hypomethylation in breast cancer: An independent parameter of tumor progression? [J].

Bernardino, T ;

Roux, C ;

Almeida, A ;

Vogt, N ;

Gibaud, A ;

GerbaultSeureau, M ;

Magdelenat, H ;

Bourgeois, CA ;

Malfoy, B ;

Dutrillaux, B .

CANCER GENETICS AND CYTOGENETICS, 1997, 97 (02) :83-89

[5] Distinctive pattern of LINE-1 methylation level in normal tissues and the association with carcinogenesis [J].

Chalitchagorn, K ;

Shuangshoti, S ;

Hourpai, N ;

Kongruttanachok, N ;

Tangkijvanich, P ;

Thong-ngam, D ;

Voravud, N ;

Sriuranpong, V ;

Mutirangura, A .

ONCOGENE, 2004, 23 (54) :8841-8846

[6] Optimization of probe length and the number of probes per gene for optimal microarray analysis of gene expression [J].

Chou, CC ;

Chen, CH ;

Lee, TT ;

Peck, K .

NUCLEIC ACIDS RESEARCH, 2004, 32 (12) :e99

[7] DNA methylation: Bisulphite modification and analysis [J].

Clark, Susan J. ;

Statham, Aaron ;

Stirzaker, Clare ;

Molloy, Peter L. ;

Frommer, Marianne .

NATURE PROTOCOLS, 2006, 1 (05) :2353-2364

[8] Quantitative data analysis methods for bead-based DNA hybridization assays using generic flow cytometry platforms [J].

Corrie, S. R. ;

Lawrie, G. A. ;

Battersby, B. J. ;

Ford, K. ;

Ruehmann, A. ;

Koehler, K. ;

Sabath, D. E. ;

Trau, M. .

CYTOMETRY PART A, 2008, 73A (05) :467-476

[9] Development of a multiplexed bead-based assay for detection of DNA methylation in cancer-related genes [J].

Corrie, Simon ;

Sova, Pavel ;

Lawrie, Gwen ;

Battersby, Bronwyn ;

Kiviat, Nancy ;

Trau, Matt .

MOLECULAR BIOSYSTEMS, 2009, 5 (03) :262-268

[10] A structural study of hybrid organosilica materials for colloid-based DNA biosensors [J].

Corrie, Simon R. ;

Vogel, Robert ;

Keen, Imelda ;

Jack, Kevin ;

Kozak, Darby ;

Lawrie, Gwendolyn A. ;

Battersby, Bronwyn J. ;

Fredericks, Peter ;

Trau, Matt .

JOURNAL OF MATERIALS CHEMISTRY, 2008, 18 (05) :523-529

← 1 2 3 →