Structure of the O-specific polysaccharide from the lipopolysaccharide of Aeromonas sobria strain Pt312

被引：6

作者：

Turska-Szewczuk, Anna ^{[1
]}

Pietras, Hubert ^{[1
]}

Duda, Katarzyna A. ^{[2
]}

Kozinska, Alicja ^{[3
]}

Pekala, Agnieszka ^{[3
]}

Holst, Otto ^{[2
]}

机构：

[1] M Curie Sklodowska Univ, Dept Genet & Microbiol, PL-20033 Lublin, Poland

[2] Leibniz Ctr Med & Biosci, Res Ctr Borstel, Div Struct Biochem, D-23845 Borstel, Germany

[3] Natl Inst Vet Res, Dept Fish Dis, PL-24100 Pulawy, Poland

来源：

CARBOHYDRATE RESEARCH | 2015年 / 403卷

关键词：

Aeromonas sobria; Fish pathogen; Lipopolysaccharide; O-specific polysaccharide; O-Acetylation; NMR; CHAIN POLYSACCHARIDE; GENUS AEROMONAS; S-LAYER; HYDROPHILA; ACETYLATION; PATHOGENICITY; SALMONICIDA; ANTIGEN; TAXONOMY; CORE;

D O I：

10.1016/j.carres.2014.06.011

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The O-specific polysaccharide (OPS) obtained by mild-acid degradation of the lipopolysaccharide from Aeromonas sobria strain Pt312 was studied by sugar and methylation analyses along with H-1 and C-13 NMR spectroscopy, including 2D H-1, H-1 COSY, TOCSY, NOESY, H-1-detected H-1, C-13 HSQC, and HMBC experiments. The sequence of the sugar residues was determined using H-1, H-1 NOESY and H-1, C-13 HMBC experiments. It was found that the OPS was built up of disaccharide repeating units composed of GlcpNAc and non-stoichiometrically O-acetylated Rhap residues, and had the structure. -> 4)-alpha-L-Rhap-(1 -> 3)-beta-D-GlcpNAc-(1 -> 2/3 | similar to 80/35% OAc (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：142 / 148

页数：7

共 40 条

[1]

Ali A, 1996, MED MICROBIOL LETT, V5, P156

[2] DISTRIBUTION OF AEROMONAS SPECIES IN WATERS WITH DIFFERENT LEVELS OF POLLUTION [J].

ARAUJO, RM ;

ARRIBAS, RM ;

PARES, R .

JOURNAL OF APPLIED BACTERIOLOGY, 1991, 71 (02) :182-186

[3] The structure of the O-specific polysaccharide from the lipopolysaccharide of Burkholderia anthina [J].

Carillo, Sara ;

Silipo, Alba ;

Perino, Vincenzo ;

Lanzetta, Rosa ;

Parrilli, Michelangelo ;

Molinaro, Antonio .

CARBOHYDRATE RESEARCH, 2009, 344 (13) :1697-1700

[4] Structure of the O-polysaccharide isolated from Cronobacter sakazakii 767 [J].

Czerwicka, Malgorzata ;

Forsythe, Stephen J. ;

Bychowska, Anna ;

Dziadziuszko, Halina ;

Kunikowska, Danuta ;

Stepnowski, Piotr ;

Kaczynski, Zbigniew .

CARBOHYDRATE RESEARCH, 2010, 345 (07) :908-913

[5] STRUCTURE OF THE HAFNIA-ALVEI STRAIN PCM-1188 O-SPECIFIC POLYSACCHARIDE [J].

GAMIAN, A ;

KATZENELLENBOGEN, E ;

ROMANOWSKA, E ;

FERNANDEZ, JMG ;

PEDERSEN, C ;

ULRICH, J ;

DEFAYE, J .

CARBOHYDRATE RESEARCH, 1995, 277 (02) :245-255

[6] Host cell invasion and intracellular residence by Aeromonas salmonicida:: Role of the S-layer [J].

Garduño, R ;

Moore, A ;

Olivier, G ;

Lizama, AL ;

Garduño, E ;

Kay, WW .

CANADIAN JOURNAL OF MICROBIOLOGY, 2000, 46 (07) :660-668

[7]

HAKOMORI SI, 1964, J BIOCHEM-TOKYO, V55, P205

[8]

HOLMBERG SD, 1986, ANN INTERN MED, V105, P683, DOI [10.7326/0003-4819-105-5-683, 10.7326/0003-4819-105-5-690]

[9] STRUCTURAL STUDIES OF THE O-SPECIFIC CHAIN AND A CORE HEXASACCHARIDE OF HAFNIA-ALVEI STRAIN-1192 LIPOPOLYSACCHARIDE [J].

JACHYMEK, W ;

PETERSSON, C ;

HELANDER, A ;

KENNE, L ;

LUGOWSKI, C ;

NIEDZIELA, T .

CARBOHYDRATE RESEARCH, 1995, 269 (01) :125-138

[10] The Genus Aeromonas: Taxonomy, Pathogenicity, and Infection [J].

Janda, J. Michael ;

Abbott, Sharon L. .

CLINICAL MICROBIOLOGY REVIEWS, 2010, 23 (01) :35-73

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