Sequencing of bacitracin A and related minor components by liquid chromatography/electrospray ionization ion trap tandem mass spectrometry

被引：29

作者：

Govaerts, C ^{[1
]}

Li, CL ^{[1
]}

Orwa, J ^{[1
]}

Van Schepdael, A ^{[1
]}

Adams, E ^{[1
]}

Roets, E ^{[1
]}

Hoogmartens, J ^{[1
]}

机构：

[1] Catholic Univ Louvain, Fac Farmaceut Wetenschappen, Lab Farmaceut Chem Anal Geneesmidelen, B-3000 Louvain, Belgium

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 2003年 / 17卷 / 12期

关键词：

D O I：

10.1002/rcm.1058

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A selective reversed-phase liquid chromatography/mass spectrometry (LC/MSn) method is described for the characterization of related compounds in commercial bacitracin samples. Mass spectral data for these polypeptide antibiotics were acquired on a LCQ ion trap mass spectrometer equipped with an electrospray ionization probe operated in the positive and negative ion mode. The LCQ ion trap is ideally suited for the sequencing of those linear side-chain cyclized peptides because it provides on-line LC/MSn capability. Using this method bacitracin A, 1-epibacitracin A, bacitracins B-1, B-2, B-3 and bacitracin F were sequenced and previous sequencing was confirmed. Bacitracins C-1, C-2, C-3, D, H-2 and H-3 were resolved chromatographically and their ring portion was sequenced for the first time. Four components not described in the literature (1-epibacitracin B-1, 1-epibacitracin B-2, 1-epibacitracin C-1 and H-4) were sequenced completely for the first time. The main advantage of this hyphenated LC/MSn technique is the characterization of the related substances without time-consuming isolation and purification procedures. Copyright (C) 2003 John Wiley Sons, Ltd.

引用

页码：1366 / 1379

页数：14

共 30 条

[1]

ABRAHAM EP, 1957, BIOCH SOME PEPTIDE S, P1

[2] CONTRIBUTIONS OF MASS-SPECTROMETRY TO PEPTIDE AND PROTEIN-STRUCTURE [J].

BIEMANN, K .

BIOMEDICAL AND ENVIRONMENTAL MASS SPECTROMETRY, 1988, 16 (1-12) :99-111

[3]

BREWER GA, 1980, ANAL PROFILES DRUG S, V9, P2

[4]

CRAIG LC, 1952, J BIOL CHEM, V199, P259

[5]

DRUCKER DB, 1987, MICROBIOLOGICAL APPL, P126

[6]

FROYSHOV O, 1984, DRUGS PHARM SCI BIOT, V22, P665

[7]

GALARDY RE, 1971, BIOCHEMISTRY-US, V10, P2429

[8]

GALLAGHER JB, 1982, J ASSOC OFF ANA CHEM, V65, P1178

[9] Characterization of polypeptide antibiotics of the polymyxin series by liquid chromatography electrospray ionization ion trap tandem mass spectrometry [J].

Govaerts, C ;

Orwa, J ;

Van Schepdael, A ;

Roets, E ;

Hoogmartens, J .

JOURNAL OF PEPTIDE SCIENCE, 2002, 8 (02) :45-55

[10] Mass spectrometric fragmentation of cyclic peptides belonging to the polymyxin and colistin antibiotics studied by ion trap and quadrupole/orthogonalacceleration time-of-flight technology [J].

Govaerts, C ;

Rozenski, J ;

Orwa, J ;

Roets, E ;

Van Schepdael, A ;

Hoogmartens, J .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2002, 16 (09) :823-833

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