DEVELOPMENT OF EST-SSR MARKERS IN COMMON FIG, FICUS CARICA L.

被引：0

作者：

Ateyyeh, Ahmad ^{[1
]}

Akash, Muhanad ^{[1
]}

Sawwan, Jamal ^{[1
]}

Abu-Rayyan, Azmi ^{[1
]}

Shiyab, Safwan ^{[1
]}

Hasan, Shireen ^{[2
]}

Al-Antary, Tawfiq M. ^{[3
]}

机构：

[1] Univ Jordan, Sch Agr, Dept Hort & Crop Sci, Amman, Jordan

[2] Univ Jordan, HMCSR, Amman, Jordan

[3] Univ Jordan, Sch Agr, Dept Plant Protect, Amman 11942, Jordan

来源：

FRESENIUS ENVIRONMENTAL BULLETIN | 2020年 / 29卷 / 06期

关键词：

BLASTX; Fig; Functional SSRs; Genetic diversity; Gene-based SSRs; Microsatellite markers; GENIC MICROSATELLITE MARKERS; TRANSFERABILITY; IDENTIFICATION; DIVERSITY; BARLEY;

D O I：

暂无

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Expressed sequence tag SSRs (EST-SSRs) are gene-derived SSRs that are easily built up using the tremendous ever-increasing cDNA clones and EST databases. Two hundred thirty eight SSR-containing EST were recognized after mining 8430 Ficus carica EST tandems that correspond approximately to 4873 Kb. A total of 360 EST-SSRs were recognized and 39 newly developed primers were designed. BLASTX identified the function of 153 (64%) EST sequences. Genetic diversity among the seven fig accessions were performed using the 22 amplified EST-SSRs. The generated dendrogram using UPGMA clustering was successful in distinguishing various fig accessions with a similarity coefficients ranged from a high of 0.93 to a low of 0.72. The present investigation is the first direct attempt to build up a large number of EST-SSR markers in Ficus carica. These EST-SSRs will not only increase the SSRs limited availability in Ficus carica but also they can be used across related species.

引用

页码：4347 / 4354

页数：8

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