Dual-colour imaging with GFP variants

被引：109

作者：

Ellenberg, J ^{[1
]}

Lippincott-Schwartz, J ^{[1
]}

Presley, JF ^{[1
]}

机构：

[1] Inst Child Hlth & Human Dev, Cell Biol & Metab Branch, Bethesda, MD 20892 USA

来源：

TRENDS IN CELL BIOLOGY | 1999年 / 9卷 / 02期

关键词：

D O I：

10.1016/S0962-8924(98)01420-2

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Green fluorescent protein(GFP) has become an important tool in cell biology and is widely used as a reporter far imaging intracellular proteins and structures in live cells. Recently, spectral variants of GFP with red- and blue-shifted fluorescence emissions have been characterized, opening the possibility of double labelling with two different-coloured GFP fusion proteins. This article reviews recent advances in this technique, with special emphasis on time-lapse imaging applications in living cells.

引用

页码：52 / 56

页数：5

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