Effect of onion-type multilamellar liposomes on Trametes versicolor laccase activity and stability

被引:13
作者
Prevoteau, Antonin [1 ]
Faure, Chrystel [1 ]
机构
[1] Univ Bordeaux, Ctr Rech Paul Pascal, CNRS, UPR8641, F-33600 Pessac, France
关键词
Multilamellar liposomes; Laccase activity; Laccase stability; NaCl effect; Temperature effect; GLUCOSE OXIDASE BIOSENSOR; ENZYME ENCAPSULATION; VESICLES; IMMOBILIZATION; NANOPARTICLES; OXIDATION; KINETICS; PHENOLS; PHASE; BEADS;
D O I
10.1016/j.biochi.2011.10.010
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Trametes versicolor laccase was encapsulated into onion-type, lipid-based multilamellar vesicles (MLVs). When encapsulated, laccase was isolated from the assay medium but was still active once freed from its capsule. The encapsulation efficiency was larger than 65% at 25 degrees C and 37 degrees C and decreased to 55% by introducing 140 mM NaCl into the buffered medium (pH = 4.5). MLVs were shown to drastically improve both laccase stability and activity. At 25 degrees C, laccase activity was doubled in the presence of MLVs. At 37 degrees C in the salt-free medium, the half-life time of laccase was increased from 2hr 30-65 h without and with MLVs, respectively. This effect was even more pronounced in the salted medium where laccase activity was unchanged for 6 days in the presence of MLVs. These beneficial effects were attributed to the immobilization of laccase onto MLV surface. Laccase activity as well as stability was notably shown to be directly correlated to MLV stability. (C) 2011 Elsevier Masson SAS. All rights reserved.
引用
收藏
页码:59 / 65
页数:7
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