cGMP and cyclic nucleotide-gated channels participate in mouse sperm capacitation

被引：14

作者：

Cisneros-Mejorado, Abraham ^{[2
]}

Sanchez Herrera, Daniel P. ^{[1
]}

机构：

[1] Ctr Invest & Estudios Avanzados IPN, Unidad Monterrey, Apodaca 66600, Nuevo Leon, Mexico

[2] Univ Guadalajara, Ctr Univ Ciencias Salud, Guadalajara 44340, Jalisco, Mexico

来源：

FEBS LETTERS | 2012年 / 586卷 / 02期

关键词：

Capacitation; Sperm; CNG channel; cGMP; EXPRESSION; MOTILITY;

D O I：

10.1016/j.febslet.2011.12.006

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

During capacitation of mammalian sperm intracellular [Ca2+] and cyclic nucleotides increase, suggesting that CNG channels play a role in the physiology of sperm. Here we study the effect of capacitation, 8Br-cAMP (8-bromoadenosine 3',5'-cyclic monophosphate) and 8Br-cGMP (8-bromoguanosine 3',5'-cyclic monophosphate) on the macroscopic ionic currents of mouse sperm, finding the existence of different populations of sperm, in terms of the recorded current and its response to cyclic nucleotides. Our results show that capacitation and cyclic nucleotides increase the ionic current, having a differential sensitivity to cGMP (cyclic guanosine monophosphate) and cAMP (cyclic adenosine monophosphate). Using a specific inhibitor we determine the contribution of CNG channels to macroscopic current and capacitation. (C) 2011 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：149 / 153

页数：5

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