A surrogate analyte-based LC-MS/MS method for the determination of 5-hydroxytryptamine, kynurenine and tryptophan

被引：11

作者：

Cong, Mengyu ^{[1
]}

Song, Jiayu ^{[1
]}

Chen, Fenglian ^{[1
]}

Cao, Cheng ^{[1
]}

Wang, Shuling ^{[1
]}

机构：

[1] Guangzhou Univ Chinese Med, Sch Chinese Pharmaceut Sci, Guangzhou, Peoples R China

来源：

BIOANALYSIS | 2020年 / 12卷 / 03期

关键词：

LC-MS; MS; surrogate analyte; tryptophan and its metabolites; LIQUID-CHROMATOGRAPHY; QUANTITATION; BRAIN; TANDEM; RAT;

D O I：

10.4155/bio-2019-0267

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Aim: The metabolism of tryptophan (TRP) through kynurenine (KYN) and 5-hydroxytryptamine (5-HT) pathways is linked to various diseases such as neurological diseases and cancer. The levels of 5-HT, KYN, TRP can be used as indicators for the diagnosis of various diseases in clinical and scientific research. Experimental: Since 5-HT, KYN, TRP are both endogenous molecules in biological samples, it is difficult to obtain a 'real blank sample'. A surrogate analyte-based LC-MS/MS method was chosen, using 5-HT-d4, KYN-d4 and TRP-d5 as surrogate analytes to replace the authentic analytes 5-HT, KYN and TRP, respectively. Theophylline was selected as the internal standard (IS). Results: The method was applied to quantification 5-HT, KYN and TRP of plasma, liver, colon, brain and verified to be acceptable in terms of linearity, precision, accuracy, matrix effect, recovery efficiency and stability.

引用

页码：129 / 142

页数：14

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