Identification and charactering of APX genes provide new insights in abiotic stresses response in Brassica napus

被引：11

作者：

Pan, Jiao ^{[1
,2
]}

Zhang, Lei ^{[1
,2
]}

Chen, Min ^{[1
,2
]}

Ruan, Yuxuan ^{[1
,2
]}

Li, Peifang ^{[1
,2
]}

Guo, Zhihui ^{[1
,2
]}

Liu, Boyu ^{[1
,2
]}

Ruan, Ying ^{[1
,2
]}

Xiao, Mu ^{[1
,2
]}

Huang, Yong ^{[1
,2
]}

机构：

[1] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha, Hunan, Peoples R China

[2] Key Lab Crop Epigenet Regulat & Dev Hunan Prov, Changsha, Hunan, Peoples R China

来源：

PEERJ | 2022年 / 10卷

关键词：

Ascorbate peroxidase; Expression pattern; Abiotic stress; Brassica napus; ASCORBATE PEROXIDASE GENE; HYDROGEN-PEROXIDE; ARABIDOPSIS; EXPRESSION; FAMILY; OVEREXPRESSION; CHLOROPLASTS; METABOLISM; TOLERANCE; EVOLUTION;

D O I：

10.7717/peerj.13166

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Ascorbate peroxidase (APX) plays an important role in scavenging H2O2 and balancing ROS content in plant cells, which is of great significance for the growth and development of life and resistance to external stress. However, up to now, APXs in Brassica napus (B. napus) have not been systematically characterized. In this study, a total of 26 BnaAPX genes were identified, which were distributed on 13 chromosomes and divided into five phylogenetic branches. Gene structure analysis showed that they had a wide varied number of exons while BnaAPXs proteins contained more similar motifs in the same phylogenetic branches. qRT-PCR analysis of 26 BnaAPX gene expression patterns showed that three putative cytosol BnaAPX genes BnaAPX1, BnaAPX2, BnaAPX9, two putatice microsomal genes BnaAPX18 and BnaAPX25 were up-regulated rapidly and robustly under high salt, water shortage and high temperature stresses. In addition, the above three abiotic stresses led to a significant increase in APX activity. The results provide basic and comprehensive information for further functional characterization of APX gene family in B. napus.

引用

页数：17

共 40 条

[1] Genome-Wide Identification and Expression Profiling of Ascorbate Peroxidase (APX) and Glutathione Peroxidase (GPX) Genes Under Drought Stress in Sorghum (Sorghum bicolor L.) [J].

Akbudak, M. Aydin ;

Filiz, Ertugrul ;

Vatansever, Recep ;

Kontbay, Kubra .

JOURNAL OF PLANT GROWTH REGULATION, 2018, 37 (03) :925-936

[2] Production and scavenging of reactive oxygen species in chloroplasts and their functions [J].

Asada, Kozi .

PLANT PHYSIOLOGY, 2006, 141 (02) :391-396

[3] Mechanism of Enhanced Superoxide Production in the Cytochrome b6f Complex of Oxygenic Photosynthesis [J].

Baniulis, Danas ;

Hasan, S. Sail ;

Stofleth, Jason T. ;

Cramer, William A. .

BIOCHEMISTRY, 2013, 52 (50) :8975-8983

[4] Plant responses to stresses: Role of ascorbate peroxidase in the antioxidant protection [J].

Caverzan, Andreia ;

Passaia, Gisele ;

Rosa, Silvia Barcellos ;

Ribeiro, Carolina Werner ;

Lazzarotto, Fernanda ;

Margis-Pinheiro, Marcia .

GENETICS AND MOLECULAR BIOLOGY, 2012, 35 (04) :1011-1019

[5] TBtools: An Integrative Toolkit Developed for Interactive Analyses of Big Biological Data [J].

Chen, Chengjie ;

Chen, Hao ;

Zhang, Yi ;

Thomas, Hannah R. ;

Frank, Margaret H. ;

He, Yehua ;

Xia, Rui .

MOLECULAR PLANT, 2020, 13 (08) :1194-1202

[6] Overexpression of the ascorbate peroxidase gene from eggplant and sponge gourd enhances flood tolerance in transgenic Arabidopsis [J].

Chiang, Chih-Ming ;

Chen, Chiu- ;

Chen, Shi-Peng ;

Lin, Kuan-Hung ;

Chen, Li-Ru ;

Su, Yu-Huei ;

Yen, His-Cheng .

JOURNAL OF PLANT RESEARCH, 2017, 130 (02) :373-386

[7] Reactive oxygen species, abiotic stress and stress combination [J].

Choudhury, Feroza K. ;

Rivero, Rosa M. ;

Blumwald, Eduardo ;

Mittler, Ron .

PLANT JOURNAL, 2017, 90 (05) :856-867

[8] Redox- and Reactive Oxygen Species-Dependent Signaling into and out of the Photosynthesizing Chloroplast [J].

Dietz, Karl-Josef ;

Turkan, Ismail ;

Krieger-Liszkay, Anja .

PLANT PHYSIOLOGY, 2016, 171 (03) :1541-1550

[9] Control of Ascorbate Peroxidase 2 expression by hydrogen peroxide and leaf water status during excess light stress reveals a functional organisation of Arabidopsis leaves [J].

Fryer, MJ ;

Ball, L ;

Oxborough, K ;

Karpinski, S ;

Mullineaux, PM ;

Baker, NR .

PLANT JOURNAL, 2003, 33 (04) :691-705

[10] Carotenoid oxidation products as stress signals in plants [J].

Havaux, Michel .

PLANT JOURNAL, 2014, 79 (04) :597-606

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