Antioxidant activity of the different polar solvent extracts of Magnolia officinalis leaves and purification of main active compounds

被引:18
作者
Tan, Li-Hong [1 ]
Zhang, Dan [1 ]
Yu, Bao [1 ]
Zhao, Sheng-Ping [1 ]
Cao, Wei-Guo [1 ,2 ]
机构
[1] Chongqing Med Univ, Coll Tradit Chinese Med, Chongqing 400016, Peoples R China
[2] Chongqing Med Univ, Lab Tradit Chinese Med, Chongqing 400016, Peoples R China
关键词
Magnolia officinalis leaves; Extraction; Purification; Antioxidant activity; Correlation analysis; KERNEL;
D O I
10.1007/s00217-014-2387-5
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
The leaf of Magnolia officinalis is abundant in natural resources as a novel potential antioxidant and has great commercial value. In this study, the contents of total phenolics, total flavonoids, magnolol and honokiol in different solvent extracts (acetone, methanol and water) were measured. Meanwhile, the antioxidant activity of extracts from M. officinalis leaves were evaluated using 1,1-diphenyl-2-picrylhydrazyl free radical scavenging assay, 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt free radical scavenging assay, FRAP assays and beta-carotene/linoleic acid system. In addition, the correlation between the antioxidant activity and the contents of the active compounds was analyzed. Experimental results indicated that three extracts exhibited antioxidant effects in a concentration-dependent manner, and acetone extracts had the highest antioxidant activity, next methanol and water. There was an intimate relativity between the contents of magnolol and honokiol in different solvent extracts and the antioxidant capability of them. So the magnolol and honokiol of M. officinalis leaves as a representative of the active compounds were extracted and purified by using the macroporous resin column. The purity of depurated sample could reach 82.6 %, which indicates the M. officinalis leaves have the potential to substitute the production of magnolol and honokiol in M. officinalis. The results of this study can provide theoretical and technical references for the reasonable use of the M. officinalis leaves and alleviate the shortage of M. officinalis resources.
引用
收藏
页码:815 / 822
页数:8
相关论文
共 16 条
[1]   Bioactive compounds and antioxidant potential of mango peel extract [J].
Ajila, C. M. ;
Naidu, K. A. ;
Bhat, S. G. ;
Rao, Uts. Prasada .
FOOD CHEMISTRY, 2007, 105 (03) :982-988
[2]  
Chang CC, 2002, J FOOD DRUG ANAL, V10, P178
[3]  
EARP CF, 1981, CEREAL CHEM, V58, P234
[4]   Oxidants, oxidative stress and the biology of ageing [J].
Finkel, T ;
Holbrook, NJ .
NATURE, 2000, 408 (6809) :239-247
[5]   Evaluation of antioxidant properties and total phenolic contents of some strains of microalgae [J].
Hajimahmoodi, Mannan ;
Faramarzi, Mohammad Ali ;
Mohammadi, Najmeh ;
Soltani, Neda ;
Oveisi, Mohammad Reza ;
Nafissi-Varcheh, Nastaran .
JOURNAL OF APPLIED PHYCOLOGY, 2010, 22 (01) :43-50
[6]   Colour, pH stability and antioxidant activity of anthocyanin rutinosides isolated from tamarillo fruit (Solanum betaceum Cav.) [J].
Hurtado, Nelson H. ;
Morales, Alicia L. ;
Lourdes Gonzalez-Miret, M. ;
Luisa Escudero-Gilete, M. ;
Heredia, Francisco J. .
FOOD CHEMISTRY, 2009, 117 (01) :88-93
[7]   Antioxidant properties of teaw (Cratoxylum formosum Dyer) extract in soybean oil and emulsions [J].
Maisuthisakul, P ;
Pongsawatmanit, R ;
Gordon, MH .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2006, 54 (07) :2719-2725
[8]   ANTIOXIDANT ACTIVITY OF PLANT-LEAVES IN RELATION TO THEIR ALPHA-TOCOPHEROL CONTENT [J].
MALLET, JF ;
CERRATI, C ;
UCCIANI, E ;
GAMISANS, J ;
GRUBER, M .
FOOD CHEMISTRY, 1994, 49 (01) :61-65
[9]   Effects of constituents from the bark of Magnolia obovata on nitric oxide production in lipopolysaccharide-activated macrophages [J].
Matsuda, H ;
Kageura, T ;
Oda, M ;
Morikawa, T ;
Sakamoto, Y ;
Yoshikawa, M .
CHEMICAL & PHARMACEUTICAL BULLETIN, 2001, 49 (06) :716-720
[10]   SIMPLIFIED METHOD FOR EVALUATION OF ANTIOXIDANTS [J].
MILLER, HE .
JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 1971, 48 (02) :91-&