Identification and Characterization of the ERF Subfamily B3 Group Revealed GhERF13.12 Improves Salt Tolerance in Upland Cotton

被引：24

作者：

Lu, Lili ^{[1
,2
]}

Qanmber, Ghulam ^{[2
]}

Li, Jie ^{[1
,2
]}

Pu, Mengli ^{[3
]}

Chen, Guoquan ^{[3
]}

Li, Shengdong ^{[3
]}

Liu, Le ^{[3
]}

Qin, Wenqiang ^{[2
]}

Ma, Shuya ^{[2
]}

Wang, Ye ^{[1
,2
]}

Chen, Quanjia ^{[1
]}

Liu, Zhao ^{[3
]}

机构：

[1] Xinjiang Agr Univ, Engn Res Ctr Cotton, Minist Educ, Urumqi, Peoples R China

[2] Chinese Acad Agr Sci, Inst Cotton Res, State Key Lab Cotton Biol, Anyang, Peoples R China

[3] Zhengzhou Univ, State Key Lab Cotton Biol, Zhengzhou Res Base, Zhengzhou, Peoples R China

来源：

FRONTIERS IN PLANT SCIENCE | 2021年 / 12卷

基金：

中国国家自然科学基金;

关键词：

ERF; phylogenetic analysis; gene duplication; collinearity analysis; ectopic expression; salt stress; gene silencing; GENOME-WIDE ANALYSIS; TRANSCRIPTION FACTOR GENE; FUNCTIONAL-ANALYSIS; ARABIDOPSIS-THALIANA; MOLECULAR-CLONING; STRESS TOLERANCE; ETHYLENE; EXPRESSION; BINDING; DROUGHT;

D O I：

10.3389/fpls.2021.705883

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The APETALA2 (AP2)/ethylene response factor plays vital functions in response to environmental stimulus. The ethylene response factor (ERF) subfamily B3 group belongs to the AP2/ERF superfamily and contains a single AP2/ERF domain. Phylogenetic analysis of the ERF subfamily B3 group genes from Arabdiposis thaliana, Gossypium arboreum, Gossypium hirsutum, and Gossypium raimondii made it possible to divide them into three groups and showed that the ERF subfamily B3 group genes are conserved in cotton. Collinearity analysis identified172 orthologous/paralogous gene pairs between G. arboreum and G. hirsutum; 178 between G. hirsutum and G. raimondii; and 1,392 in G. hirsutum. The GhERF subfamily B3 group gene family experienced massive gene family expansion through either segmental or whole genome duplication events, with most genes showing signature compatible with the action of purifying selection during evolution. Most G. hirsutum ERF subfamily B3 group genes are responsive to salt stress. GhERF13.12 transgenic Arabidopsis showed enhanced salt stress tolerance and exhibited regulation of related biochemical parameters and enhanced expression of genes participating in ABA signaling, proline biosynthesis, and ROS scavenging. In addition, the silencing of the GhERF13.12 gene leads to increased sensitivity to salt stress in cotton. These results indicate that the ERF subfamily B3 group had remained conserved during evolution and that GhERF13.12 induces salt stress tolerance in Arabidopsis and cotton.

引用

页数：15

共 100 条

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