Real-time imaging of lymphocytes in vivo

被引：69

作者：

Cahalan, MD ^{[1
]}

Parker, I

Wei, SH

Miller, MJ

机构：

[1] Univ Calif Irvine, Dept Physiol & Biophys, Irvine, CA 92697 USA

[2] Univ Calif Irvine, Dept Neurobiol & Behav, Irvine, CA 92697 USA

来源：

CURRENT OPINION IN IMMUNOLOGY | 2003年 / 15卷 / 04期

关键词：

D O I：

10.1016/S0952-7915(03)00079-7

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

New preparations, fluorescent probes and imaging techniques are providing the means to observe the behavior of cells in the tissue environment of lymphoid organs. In particular, when combined with two-photon laser microscopy, intravital imaging of surgically exposed lymph nodes provides a unique view of lymphocyte migration and antigen presentation as it occurs within the living animal. The view is emerging that lymphocytes migrate randomly within lymphoid organs, and that lymphocyte contact with antigen-presenting cells may be a stochastic process rather than one guided by chemokine gradients.

引用

页码：372 / 377

页数：6

共 25 条

[1] Dynamics of CD8+ T cell priming by dendritic cells in intact lymph nodes [J].

Bousso, P ;

Robey, E .

NATURE IMMUNOLOGY, 2003, 4 (06) :579-585

[2] Dynamics of thymocyte-stromal cell interactions visualized by two-photon microscopy [J].

Bousso, P ;

Bhakta, NR ;

Lewis, RS ;

Robey, E .

SCIENCE, 2002, 296 (5574) :1876-1880

[3] Two-photon tissue imaging: Seeing the immune system in a fresh light [J].

Cahalan, MD ;

Parker, I ;

Wei, SH ;

Miller, MJ .

NATURE REVIEWS IMMUNOLOGY, 2002, 2 (11) :872-880

[4] Differential effects of physiologically relevant hypoxic conditions on T lymphocyte development and effector functions [J].

Caldwell, CC ;

Kojima, H ;

Lukashev, D ;

Armstrong, J ;

Farber, M ;

Apasov, SG ;

Sitkovsky, MV .

JOURNAL OF IMMUNOLOGY, 2001, 167 (11) :6140-6149

[5] Advances in vivo bioluminescence imaging of gene expression [J].

Contag, CH ;

Bachmann, MH .

ANNUAL REVIEW OF BIOMEDICAL ENGINEERING, 2002, 4 :235-260

[6] Chemokines - Chemokines and cell migration in secondary lymphoid organs [J].

Cyster, JG .

SCIENCE, 1999, 286 (5447) :2098-2102

[7] Imaging of T-cell interactions with antigen presenting cells in culture and in intact lymphoid tissue [J].

Delon, J ;

Stoll, S ;

Germain, RN .

IMMUNOLOGICAL REVIEWS, 2002, 189 :51-63

[8] Detection of single mammalian cells by high-resolution magnetic resonance imaging [J].

Dodd, SJ ;

Williams, M ;

Suhan, JP ;

Williams, DS ;

Koretsky, AP ;

Ho, C .

BIOPHYSICAL JOURNAL, 1999, 76 (01) :103-109

[9] Quantitative imaging of the T cell antitumor response by positron-emission tomography [J].

Dubey, P ;

Su, H ;

Adonai, N ;

Du, SY ;

Rosato, A ;

Braun, J ;

Gambhir, SS ;

Witte, ON .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (03) :1232-1237

[10] Reprograming T cells: the role of extracellular matrix in coordination of T cell activation and migration [J].

Dustin, ML ;

de Fougerolles, AR .

CURRENT OPINION IN IMMUNOLOGY, 2001, 13 (03) :286-290

← 1 2 3 →