A novel toti-like virus from a plant pathogenic oomycete Globisporangium splendens

被引：10

作者：

Shiba, Kazuki ^{[1
]}

Hatta, Chiharu ^{[1
]}

Sasai, Shinsaku ^{[1
]}

Tojo, Motoaki ^{[1
]}

Ohki, Satoshi T. ^{[1
]}

Mochizuki, Tomofumi ^{[1
]}

机构：

[1] Osaka Prefecture Univ, Grad Sch Life & Environm Sci, Sakai, Osaka 5998531, Japan

来源：

VIROLOGY | 2019年 / 537卷

关键词：

Globisporangium splendens; Next generation sequencing; Oomycete virus; Pythium; Totivirus; Vertical transmission; DOUBLE-STRANDED-RNA; PERFORMANCE; ALGORITHMS;

D O I：

10.1016/j.virol.2019.08.025

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We investigated virus infection in the plant pathogenic oomycete Globisporangium splendens, formerly classified as Pythium splendens, in Japan. From 12 strains investigated, three strains contained virus-like double-stranded (dsRNA). Next-generation sequencing revealed that the G. splendens strain MAFF 425508 and MAFF 305867 contained a virus related to toti-like viruses, that we named Pythium splendens RNA virus 1 (PsRV1). PsRV1 has a ca. 5700 nt-length genome encoding two overlapping open reading frames (ORFs). The ORF2 encodes an RNA-dependent RNA polymerase (RdRp). Phylogenetic analysis with deduced RdRp amino acid sequences indicated that PsRV1 was closely related to Pythium polare RNA virus 1 (PpRV1) from G. polare infecting mosses in the Arctic. PsRV1 was vertically transmitted through the hyphal swellings, vegetative organs of G. splendens, in a temperature-dependent manner. Also, we showed that PsRV1 infected in a symptomless manner.

引用

页码：165 / 171

页数：7

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