Circulating skeletal stem cells

被引：523
作者：
Kuznetsov, SA
Mankani, MH
Gronthos, S
Satomura, K
Bianco, P
Robey, PG
机构：
[1] Natl Inst Dent & Craniofacial Res, Craniofacial & Skeletal Dis Branch, NIH, Bethesda, MD 20892 USA
[2] Univ Tokushima, Sch Dent, Dept Oral & Maxillofacial Surg 1, Tokushima 7708504, Japan
[3] Univ Roma La Sapienza, Dipartimento Med Sperimental & Patol, I-00161 Rome, Italy
来源：
JOURNAL OF CELL BIOLOGY | 2001年 / 153卷 / 05期
关键词：
blood cells; tissue culture; adherent colonies; transplantation; osteogenesis;
D O I：
10.1083/jcb.153.5.1133
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
We report the isolation of adherent, clonogenic, fibroblast-like cells with osteogenic and adipogenic potential from the blood of four mammalian species. These cells phenotypically resemble but are distinguishable from skeletal stem cells found in bone marrow (stromal stem cells,''mesenchymal stem cells"), The osteogenic potential of the blood-borne cells was proven by an in vivo transplantation assay in which either polyclonal or single colony-derived strains were trans-planted into the subcutis of immunocompromised mice, and the donor origin of the fully differentiated bone cells was proven using species-specific probes. This is the first definitive proof of the existence of circulating skeletal stem cells in mammals.
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页码：1133 / 1139
页数：7
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