Synthesis and protein binding properties of novel highly functionalized Calix[4]arene

被引：7

作者：

Zadmard, Reza ^{[1
]}

Darvishi, Shukufe ^{[1
]}

Akbari-Moghaddam, Peyman ^{[1
]}

机构：

[1] CCERCI, POB 14335-186, Tehran, Iran

来源：

JOURNAL OF INCLUSION PHENOMENA AND MACROCYCLIC CHEMISTRY | 2016年 / 86卷 / 1-2期

关键词：

Calix[4]arene; Protein; Fluorescence; Lysozyme; Binding affinity; BOVINE SERUM-ALBUMIN; TRYPTOPHAN FLUORESCENCE; MULTICOMPONENT REACTION; IN-VITRO; RECOGNITION; CALIXARENES; CONFORMATION; DERIVATIVES; ADAPTATION; INHIBITOR;

D O I：

10.1007/s10847-016-0637-9

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A novel protein binding agent based on highly functionalized calix[4]arene 5 has been synthesized and its structure characterized by H-1 and C-13 NMR, FT-IR and HRMS spectral data. Protein binding studies carried out using fluorescence spectroscopic technique revealed that this compound could bind strongly to lysozyme. Furthermore, the effect of compound 5 on the conformation of lysozyme was investigated using three-dimensional fluorescence spectroscopy. Finally, the influence of various biological relevant metal ions on the binding constant of lysozyme with 5 was studied.

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收藏

页码：27 / 32

页数：6

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