Immunochromatographic assay for T-2 toxin based on luminescent quantum dot beads

被引：8

作者：

Qie, Zhiwei ^{[1
]}

Shi, Jinmiao ^{[1
]}

Yan, Wenliang ^{[1
]}

Gao, Zichen ^{[2
]}

Meng, Wu ^{[2
]}

Xiao, Rui ^{[1
]}

Wang, Shengqi ^{[1
]}

机构：

[1] Beijing Inst Radiat Med, Beijing 100850, Peoples R China

[2] Ctr Dis Prevent & Control Rocket Force, Beijing, Peoples R China

来源：

RSC ADVANCES | 2019年 / 9卷 / 66期

关键词：

LINKED-IMMUNOSORBENT-ASSAY; MASS-SPECTROMETRY METHOD; QUANTITATIVE DETECTION; LIQUID-CHROMATOGRAPHY; HT-2; TOXINS; VALIDATION; TRICHOTHECENES; APTASENSOR; RESIDUES; SENSOR;

D O I：

10.1039/c9ra06689f

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A quantum dot bead based immunochromatographic assay (QB-ICA) system was established for T-2 toxin (T-2), which widely occurs in agriculture and could be used as a potential biological warfare agent. After optimization, the dynamic linear detection range of T-2 calculated from a calibration curve was from 0.12 to 0.67 ng mL(-1) and the limit of detection (LOD) was 0.08 ng mL(-1), which is lower than those of the ICA based on colloidal gold nanoparticles or a fluorescent material or an antibody-based biochip in other reports. The performance and practicability of the established ICA system were validated with a commercial ELISA kit and the two methods were comparable. The proposed QB-ICA for T-2 could be an alternative for rapid, sensitive, and quantitative on-site detection of this toxin in biosafety monitoring in agriculture and for susceptibility testing of the potential release of this biological warfare agent.

引用

页码：38697 / 38702

页数：6

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