Characterization of a long isoform of IL-1R8 (TIR8/SIGIRR)

被引：12
作者：
Vilia, Maria Giovanna [1 ]
Tocchetti, Marta [1 ]
Fonte, Eleonora [1 ]
Sana, Ilenia [1 ]
Muzio, Marta [1 ]
机构：
[1] Osped San Raffaele, IRCCS, Div Expt Oncol, Cell Signaling Unit, Via Olgettina 58, Milan, Italy
来源：
EUROPEAN CYTOKINE NETWORK | 2017年 / 28卷 / 02期
关键词：
interleukin-1; receptor; Toll-like receptor; IL-1R8; TIR8; SIGIRR; splicing; TOLL-LIKE RECEPTORS; INTERLEUKIN-1; RECEPTOR; SIGNAL-TRANSDUCTION; INNATE IMMUNITY; IL-37; REQUIRES; MESSENGER-RNA; SIGIRR; INFLAMMATION; FAMILY; INHIBITION;
D O I：
10.1684/ecn.2017.0395
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
IL-1R8, also known as SIGIRR or TIR8, is a trans-membrane protein belonging to the IL-1 receptor family. The human gene includes ten exons, and alternative splicing can result in different isoforms. We, herein, characterized a longer isoform of IL-1R8 containing an in-frame additional sequence between the TIR domain and the C-terminal portion of the protein. IL-1R8 Long (IL-1R8L1) mRNA was specifically expressed and regulated in distinct cell lines, in a manner similar to the classic isoform. Overexpression of IL-1R8L1 resulted in the production of a corresponding protein that showed a pattern of cell localization similar to the classic isoform. An antibody directed against an IL-1R8L1 specific peptide, detected this novel isoform in different cell lines and tissues where this protein may complement the anti-inflammatory functions of classic IL-1R8.
引用
收藏
页码：63 / 69
页数：7
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