Identification of proximal SUMO-dependent interactors using SUMO-ID

被引：34

作者：

Barroso-Gomila, Orhi ^{[1
]}

Trulsson, Fredrik ^{[2
]}

Muratore, Veronica ^{[1
]}

Canosa, Inigo ^{[1
]}

Merino-Cacho, Laura ^{[1
]}

Rosa Cortazar, Ana ^{[1
,3
]}

Perez, Coralia ^{[1
]}

Azkargorta, Mikel ^{[1
,4
,5
]}

Iloro, Ibon ^{[1
,4
,5
]}

Carracedo, Arkaitz ^{[1
,3
,6
,7
]}

Aransay, Ana M. ^{[1
,4
]}

Elortza, Felix ^{[1
,4
,5
]}

Mayor, Ugo ^{[6
,7
]}

Vertegaal, Alfred C. O. ^{[2
]}

Barrio, Rosa ^{[1
]}

Sutherland, James D. ^{[1
]}

机构：

[1] Ctr Cooperat Res Biosci CIC bioGUNE, Basque Res & Technol Alliance BRTA, Bizkaia Technol Pk,Bldg 801 A, Derio 48160, Spain

[2] Leiden Univ Med Ctr LUMC, Cell & Chem Biol, NL-2333 ZA Leiden, Netherlands

[3] Inst Salud Carlos III, CIBERONC, C Monforte de Lemos 3-5,Pabellon 11,Planta 0, Madrid 28029, Spain

[4] Inst Salud Carlos III, CIBERehd, C Monforte de Lemos 3-5,Pabellon 11,Planta 0, Madrid 28029, Spain

[5] Inst Salud Carlos III, ProteoRed ISCIII, C Monforte de Lemos 3-5,Pabellon 11,Planta 0, Madrid 28029, Spain

[6] Basque Fdn Sci, Ikerbasque, Bilbao 48011, Spain

[7] Univ Basque Country, UPV EHU, Biochem & Mol Biol Dept, E-48940 Leioa, Spain

来源：

NATURE COMMUNICATIONS | 2021年 / 12卷 / 01期

基金：

欧洲研究理事会;

关键词：

PML NUCLEAR-BODIES; PROMYELOCYTIC LEUKEMIA; WEB-SERVER; RAR-ALPHA; SUMOYLATION; UBIQUITIN; REVEALS; BODY; PROTEINS; SALL1;

D O I：

10.1038/s41467-021-26807-6

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The fast dynamics and reversibility of posttranslational modifications by the ubiquitin family pose significant challenges for research. Here we present SUMO-ID, a technology that merges proximity biotinylation by TurboID and protein-fragment complementation to find SUMO-dependent interactors of proteins of interest. We develop an optimized split-TurboID version and show SUMO interaction-dependent labelling of proteins proximal to PML and RANGAP1. SUMO-dependent interactors of PML are involved in transcription, DNA damage, stress response and SUMO modification and are highly enriched in SUMO Interacting Motifs, but may only represent a subset of the total PML proximal proteome. Likewise, SUMO-ID also allow us to identify interactors of SUMOylated SALL1, a less characterized SUMO substrate. Furthermore, using TP53 as a substrate, we identify SUMO1, SUMO2 and Ubiquitin preferential interactors. Thus, SUMO-ID is a powerful tool that allows to study the consequences of SUMO-dependent interactions, and may further unravel the complexity of the ubiquitin code.

引用

页数：19

共 82 条

[1] Heterologous SUMO-2/3-Ubiquitin Chains Optimize IκBα Degradation and NF-κB Activity [J].