Molecular identification and further characterization of Arcanobacterium pyogenes isolated from bovine mastitis and from various other origins

被引：57

作者：

Hijazin, M. ^{[1
]}

Uelbegi-Mohyla, H. ^{[1
]}

Alber, J. ^{[1
]}

Laemmler, C. ^{[1
]}

Hassan, A. A. ^{[2
]}

Abdulmawjood, A. ^{[3
]}

Prenger-Berninghoff, E. ^{[4
]}

Weiss, R. ^{[4
]}

Zschoeck, M. ^{[5
]}

机构：

[1] Univ Giessen, Inst Pharmakol & Toxikol, D-35392 Giessen, Germany

[2] GD Anim Hlth Serv, Dept Bacteriol & Parasitol, NL-7400 AA Deventer, Netherlands

[3] Univ Giessen, Inst Tierarztliche Nahrungsmittelkunde, D-35392 Giessen, Germany

[4] Univ Giessen, Inst Hyg & Infekt Krankheiten Tiere, D-35392 Giessen, Germany

[5] Landesbetrieb Hess Landeslab, D-35392 Giessen, Germany

来源：

JOURNAL OF DAIRY SCIENCE | 2011年 / 94卷 / 04期

关键词：

Arcanobacterium pyogenes; mastitis; molecular identification; virulence gene; COLLAGEN-BINDING PROTEIN; ACTINOMYCES PYOGENES; GENE; NEURAMINIDASE; HEMOLYSIN; UTERUS; CBPA;

D O I：

10.3168/jds.2010-3678

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

The present study was designed to identify phenotypically and genotypically 61 Arcanobacterium pyogenes isolated from bovine mastitis and, from various other origins. The A. pyogenes isolates showed the typical cultural and biochemical properties of this species and displayed CAMP-like synergistic hemolytic activities with various indicator strains. The species identity could be confirmed genotypically by amplification and sequencing of the superoxide dismutase A encoding gene sodA of reference strains representing 8 species of genus Arcanobacterium and subsequent design of A. pyogenes sodA gene-specific oligonucleotide primer. The A. pyogenes sodA gene-specific oligonucleotide primer allowed, together with previously described A. pyogenes 16S-23S rDNA intergenic spacer region-specific oligonucleotide primer, a reliable molecular identification of all 61 A. pyogenes of various origins. The additionally performed PCR-mediated amplification of 5 known and putative virulence factor encoding genes revealed that 100, 20, 87, 75, and 98% of the A. pyogenes carried the genes plo, cbpA, nanP, and fimA, which allowed an individual strain characterization. This might help to elucidate the role the putative virulence factors play in bovine mastitis and in various other infections caused by this bacterial pathogen.

引用

页码：1813 / 1819

页数：7

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