An artificial CO-releasing metalloprotein built by histidine-selective metallation

被引：19

作者：

Albuquerque, Ines S. ^{[1
]}

Jeremias, Helia F. ^{[2
]}

Chaves-Ferreira, Miguel ^{[1
]}

Matak-Vinkovic, Dijana ^{[3
]}

Boutureira, Omar ^{[3
]}

Romao, Carlos C. ^{[2
]}

Bernardes, Gonalo J. L. ^{[1
,3
]}

机构：

[1] Univ Lisbon, Fac Med, Inst Mol Med, P-1649028 Lisbon, Portugal

[2] Univ Nova Lisboa, Inst Tecnol Quim & Biol Antonio Xavier, P-2780157 Oeiras, Portugal

[3] Univ Cambridge, Dept Chem, Cambridge CB2 1EW, England

来源：

CHEMICAL COMMUNICATIONS | 2015年 / 51卷 / 19期

基金：

英国工程与自然科学研究理事会;

关键词：

CARBON-MONOXIDE; RUTHENIUM COMPLEXES; IN-VIVO; METALLOENZYMES; INTERLEUKIN-8; DESIGN; REACTIVITY; MOLECULES; PROTEINS; DELIVERY;

D O I：

10.1039/c4cc10204e

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We report the design and synthesis of an aquacarbonyl Ru(II) dication cis-[Ru(CO)(2)(H2O)(4)](2+) reagent for histidine (His)-selective metallation of interleukin (IL)-8 at site 33. The artificial, non-toxic interleukin (IL)-8-Ru-II(CO)(2) metalloprotein retained IL-8-dependent neutrophil chemotactic activity and was shown to spontaneously release CO in live cells.

引用

页码：3993 / 3996

页数：4

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