PREPARATION OF QUERCETIN-Cu(II) COMPLEX MOLECULARLY IMPRINTED POLYMER AND ITS BINDING CHARACTERISTICS

A series of complex molecularly imprinted polymers were prepared in THF/methand mixed solvents, using quercetin-Cu (II) complex as template and methacrylic acid as functional monomer. The interaction between quercetin (quercetin is a kind of flavonoids which are important active ingredients of Chinese herbs) and Cu (II) was investigated by UV-Vis spectroscopy, which reveals the formation of 1: 2 complex in the system. The results of UV-Vis spectroscopy also indicated the interaction among quercetin, Cu(II) and methacrylic acid. The effect of solvent on the morphology and network structure of complex molecularly imprinted polymer and its binding capacity were studied by transmission electron microscopy and equilibrium binding experiments. It turned out that binding capacity was greatly influenced by solvent amount. Systematic study on recognition properties of imprinted polymer was carried out and effect of different anions and cations on binding capacities of complex molecularly imprinted polymer was investigated. It was found that complex molecularly imprinted polymer showed good binding characteristics to quercetin in the presence of Cu(II) and the max binding amount reached 61.09 mu mol/g. Besides quercetin-Cu(II) complex molecularly imprinted polymer has excellent elution property, and it is easy to be regenerated and reused.