Extracellular cations sensitize and gate capsaicin receptor TRPV1 modulating pain signaling

被引:142
作者
Ahern, GP [1 ]
Brooks, IM [1 ]
Miyares, RL [1 ]
Wang, XB [1 ]
机构
[1] Georgetown Univ, Dept Pharmacol, Washington, DC 20007 USA
关键词
TRPV1; VR1; nociception; strength; pain;
D O I
10.1523/JNEUROSCI.0237-05.2005
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Transient receptor potential (TRP) channels detect diverse sensory stimuli, including alterations in osmolarity. However, a molecular detector of noxious hypertonic stimuli has not yet been identified. We show here that acute pain-related behavior evoked by elevated ionic strength is abolished in TRP vanilloid subtype 1 ( TRPV1)-null mice and inhibited by iodoresiniferatoxin, a potent TRPV1 antagonist. Electrophysiological recordings demonstrate a novel form of ion channel modulation by which extracellular Na+, Mg2+, and Ca2+ ions sensitize and activate the capsaicin receptor, TRPV1. At room temperature, increasing extracellular Mg2+ ( from 1 to 5mM) or Na+ (+50 mM) increased ligand-activated currents up to fourfold, and 10mM Mg2+ reduced the EC50 for activation by capsaicin from 890 to 450 nM. Moreover, concentrations of divalent cations >10mM directly gate the receptor. These effects occur via electrostatic interactions with two glutamates (E600 and E648) formerly identified as proton-binding residues. Furthermore, phospholipase C-mediated signaling enhances the effects of cations, and physiological concentrations of cations contribute to the bradykinin-evoked activation of TRPV1 and the sensitization of the receptor to heat. Thus, the modulation of TRPV1 by cationic strength may contribute to inflammatory pain signaling.
引用
收藏
页码:5109 / 5116
页数:8
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