Design, Synthesis, Characterization and Biological Evaluation of Ruthenium Complexes of Flavonethiosemicarbazones as Antiproliferative and Mycobacterial Activities

The ruthenium(II) complexes of 3-hydroxy-4'-substituted flavonethiosemicarbazones, (where 4'-substituents are -OCH3 (LB1), -Cl (LB2), NMe2 (LB3)) with Ru(bpy)(2)Cl-2 and Ru (phen)(2)Cl-2 (bpy=2, 2 ' bipyridine; Phen=1, 10 phenanthroline) viz. [Ru(bpy)(2)(LB1)]Cl-2 (MB1), [Ru(bpy)(2)(LB2)]Cl-2.2H(2)O, (MB2), [Ru(bpy)(2)(LB3)]Cl-2.H2O (MB3), [Ru(phen)(2)(LB1)]Cl-2.H2O, (MP1), [Ru(phen)(2)(LB2)]Cl-2 center dot 1.5H(2)O (MP2), [Ru(phen)(2)(LB3)]Cl-2 (MP3) were synthesized in good yield. All the compounds were characterized by elemental analysis, IR, H-1 NMR, UV/Vis spectroscopy and ESI-MS. The spectral features of the complexes matches well to their corresponding formula. The gas phase molecular structures of all complexes were determined using density functional theory (DFT) calculations. All the compounds were screened on A549 (Human Lung carcinoma) cell line using the MTT cell viability assay. The anti-mycobacterial drug susceptibility testing (DST) was performed using Resazurin Microtiter plate assay with glycerol as carbon source. The effect of compounds on morphological changes of cultured cells was analysed. The compound MB2 showed good cytotoxic activity against human lung carcinoma cell line A549. Similarly, compound MP2 showed good anti-mycobacterial activity with more than 40% inhibition of growth.